"471649" . "15110" . . . . "Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kB in HeLa cells"@en . "Ulrichov\u00E1, Jitka" . . "Natural compounds have been used in the treatment of various diseases for centuries. Herein, we investigated the effects of structurally diverse alkaloids with anti-inflammatory activity (berberine, sanguinarine, chelerythrine, and colchicine) on two important anti-inflammatory and pro-inflammatory players, i.e. glucocorticoid receptor (GR) and nuclear factor kappa B (NF-\u0119B), respectively. Sanguinarine and chelerythrine elicited nuclear translocation of the p65 subunit of NF-\u0119B. The nuclear import of p65 was strongly augmented by these akaloids in non-stimulated cells as well as in cells challenged with tumor necrosis factor alpha (TNF\u00E1). Colchicine and berberine had no effect on p65 nuclear translocation regardless of the presence or absence of TNF\u00E1. Colchicine caused rapid degradation of the GR protein, whereas berberine had no effect on GR content or cellular localization. Sanguinarine and chelerythrine induced accumulation of GR in the nucleus with concomitant diminution of cytosolic GR. Analyses" . "Chemico-Biological Interactions" . . "Vrzal, Radim" . "Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kB in HeLa cells" . "2" . . "12"^^ . "Berberine; colchicine; sanguinarine; chelerythrine; glucocorticoid receptor; nuclear factor kappa B; HeLa cells"@en . "RIV/61989592:15110/06:00006983" . . . . "0009-2797" . "IE - Irsko" . . "P(GP303/04/P074), Z(MSM6198959216)" . . . "2"^^ . "Maurel, Patrick" . "[73597587745D]" . . "Natural compounds have been used in the treatment of various diseases for centuries. Herein, we investigated the effects of structurally diverse alkaloids with anti-inflammatory activity (berberine, sanguinarine, chelerythrine, and colchicine) on two important anti-inflammatory and pro-inflammatory players, i.e. glucocorticoid receptor (GR) and nuclear factor kappa B (NF-\u0119B), respectively. Sanguinarine and chelerythrine elicited nuclear translocation of the p65 subunit of NF-\u0119B. The nuclear import of p65 was strongly augmented by these akaloids in non-stimulated cells as well as in cells challenged with tumor necrosis factor alpha (TNF\u00E1). Colchicine and berberine had no effect on p65 nuclear translocation regardless of the presence or absence of TNF\u00E1. Colchicine caused rapid degradation of the GR protein, whereas berberine had no effect on GR content or cellular localization. Sanguinarine and chelerythrine induced accumulation of GR in the nucleus with concomitant diminution of cytosolic GR. Analyses"@en . "RIV/61989592:15110/06:00006983!RIV09-MSM-15110___" . . "Rozd\u00EDln\u00E9 \u00FA\u010Dinky vybran\u00FDch p\u0159\u00EDrodn\u00EDch l\u00E1tek s protiz\u00E1n\u011Btlivou aktivitou na glukokortikoidn\u00ED receptor a NF-kB v HeLA bu\u0148k\u00E1ch"@cs . . . "4"^^ . . "Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kB in HeLa cells" . . "Dvo\u0159\u00E1k, Zden\u011Bk" . . "Rozd\u00EDln\u00E9 \u00FA\u010Dinky vybran\u00FDch p\u0159\u00EDrodn\u00EDch l\u00E1tek s protiz\u00E1n\u011Btlivou aktivitou na glukokortikoidn\u00ED receptor a NF-kB v HeLA bu\u0148k\u00E1ch"@cs . . "P\u0159\u00EDrodn\u00ED l\u00E1tky byly pou\u017E\u00EDv\u00E1ny v l\u00E9\u010Db\u011B r\u016Fzn\u00FDch onemocn\u011Bn\u00ED po stalet\u00ED. V t\u00E9to pr\u00E1ci jsme studoval \u00FA\u010Dinek strukturn\u011B odli\u0161n\u00FDch alkaloid\u016F s protiz\u00E1n\u011Btlivou aktivitou (berberinu, sanguinarinu, chelerythrinu a kolchicinu) na dva d\u016Fle\u017Eit\u00E9 protiz\u00E1n\u011Btliv\u00E9 a proz\u00E1n\u011Btliv\u00E9 faktory, tj. glukokortikoidn\u00ED receptor (GR) a nukle\u00E1rn\u00ED faktor kappa B (NFkB). Sanguinarin a chelerythrin vyvolaly jadernou translokaci p65 podjednotky faktoru NFkB. Jaden\u00FD import p65 byl t\u011Bmito alkaloidy v\u00FDrazn\u011B zes\u00EDlen v nestimulovan\u00FDch bu\u0148k\u00E1ch stejn\u011B tak jako v bu\u0148k\u00E1ch inkubovan\u00FDch s tumor nekrotizuj\u00EDc\u00EDm faktorem alfa (TNFa). Kolchicin a berberin nem\u011Bly \u017E\u00E1dn\u00FD \u00FA\u010Dinek na jadernou translokaci p65 bez ohledu na p\u0159\u00EDtomnost nebo nep\u0159\u00EDtomnost TNFa. Kolchicin zp\u016Fsobil rychlou degradaci GR proteinu, zat\u00EDmco berberin nem\u011Bl vliv na obsah a bun\u011B\u010Dnou lokalizaci GR. Sanguinarin a chelerythrin indukovaly akumulaci GR v j\u00E1d\u0159e a sou\u010Dasn\u011B vymizen\u00ED cytosolick\u00E9ho GR. Anal\u00FDzami transkrip\u010Dn\u00EDch aktivit GR a NFkB report\u00E9rov\u00FDmi zkou\u0161kami v HeLa bu\u0148k\u00E1ch transientn\u011B t"@cs . . "159" . . "Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kB in HeLa cells"@en .