"Porovn\u00E1n\u00ED vlivu chelerythrinu a sanguinarine na bun\u011B\u010Dn\u00FD r\u016Fst, apopt\u00F3zu a bun\u011B\u010Dn\u00FD cyklus u lidsk\u00FDch gingiv\u00E1ln\u00EDch fibroblast\u016F a n\u00E1dorov\u00FDch bun\u011Bk"@cs . "Apoptosis; Cell cycle; Chelerythrine; Cytotoxicity; DNA damage; Sanguinarine"@en . "Z(MSM6198959216)" . . "We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural mol" . . . "The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine" . . "[1D87BF8EDFEB]" . . "Ulrichov\u00E1, Jitka" . . "V t\u00E9to studii jsme porovn\u00E1vali vliv chelerythrinu (CHE) a sanguinarinu (SA) na lidsk\u00E9 prostatick\u00E9 n\u00E1dorov\u00E9 bun\u011B\u010Dn\u00E9 linie (LNCaP a DU-145) a prim\u00E1rn\u00ED kulturu lidsk\u00FDch gingiv\u00E1ln\u00EDch fibroblast\u016F. Bu\u0148ky byly vystaveny 24 h p\u016Fsoben\u00ED SA a CHE. Pot\u00E9 bylo pozorov\u00E1no (1) sn\u00ED\u017Een\u00ED \u017Eivotnosti bun\u011Bk v z\u00E1vislosti na koncentraci SA a CHE u v\u0161ech testovan\u00FDch bun\u011B\u010Dn\u00FDch model\u016F (hodnoceno MTT testem a inkorporac\u00ED bromdeoxyuridinu do bun\u011Bk); (2) n\u00E1r\u016Fst po\u0161kozen\u00ED DNA v z\u00E1vislosti na koncentraci l\u00E1tek u v\u0161ech testovan\u00FDch bun\u011Bk (hodnoceno metodou Comet essay); (3) zm\u011Bny v hladin\u00E1ch protein\u016F zapojen\u00FDch do procesu apopt\u00F3zy (sledovan\u00E9 western blot anal\u00FDzou a metodou TUNEL); (4) signifikantn\u00ED indukce inhibitor\u016F cyklin-dependentn\u00EDch kinas p21Waf1/Cip1 a p27Kip1 u prostatick\u00FDch n\u00E1dorov\u00FDch bun\u011B\u010Dn\u00FDch lini\u00ED (stanoven\u00E9 western blot anal\u00FDzou). V t\u00E9to studii jsme dok\u00E1zaly, \u017Ee CHE m\u00E1 statisticky v\u00FDznamn\u00FD cytotoxick\u00FD \u00FA\u010Dinek na prostatick\u00E9 n\u00E1dorov\u00E9 bun\u011B\u010Dn\u00E9 linie, nez\u00E1visl\u00FD na obsahu proteinu p53 a androgenu. Norm\u00E1ln\u00ED gingiv\u00E1ln\u00ED fibroblast"@cs . "Mal\u00EDkov\u00E1, Jana" . "22" . "RIV/61989592:15110/06:00003481!RIV07-MSM-15110___" . "RIV/61989592:15110/06:00003481" . . . "The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine"@en . "The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine"@en . "15"^^ . "Porovn\u00E1n\u00ED vlivu chelerythrinu a sanguinarine na bun\u011B\u010Dn\u00FD r\u016Fst, apopt\u00F3zu a bun\u011B\u010Dn\u00FD cyklus u lidsk\u00FDch gingiv\u00E1ln\u00EDch fibroblast\u016F a n\u00E1dorov\u00FDch bun\u011Bk"@cs . . . "4"^^ . "4"^^ . . . "Zda\u0159ilov\u00E1, Ad\u00E9la" . . . . . . "472985" . "We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural mol"@en . . "Cell Biology and Toxicology" . "15110" . "439-453" . "The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine" . "NL - Nizozemsko" . "Hlobilkov\u00E1, Alice" . . . "0742-2091" . "6" . .