. "6"^^ . "Cytotoxicita sanguinarinu v prim\u00E1rn\u00EDch potkan\u00EDch hepatocytech je sn\u00ED\u017Eena dioxinem a fenobarbitalem"@cs . "5"^^ . . . . "Anzenbacherov\u00E1, Eva" . . "Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital"@en . "Z(MSM6198959216)" . . "RIV/61989592:15110/06:00003478!RIV07-MSM-15110___" . . "Sanguinarine; Cytochrome P450; Induction; Cytotoxicity"@en . . "Putative interactions between quaternary benzo[c]phenanthridine alkaloid sanguinarine (SA) and aryl hydrocarbon receptor/cytochrome P450 CYP1A (AhR/CYP1A) regulatory pathway are the subject of perpetual disputations. The role of CYP1A enzymes and AhR receptor in SA cytotoxicity was anticipated. In this paper, we tested, whether selected inducers of CYP enzymes modulate cytotoxicity of SA in primary cultures of rat hepatocytes. Cells were challenged 48h with dioxin (TCDD; 5nM), phenobarbital (PB; 500microM) or DMSO prior to the treatment with SA. SA itself displayed time- and dose-dependent cytotoxicity as revealed by lactate dehydrogenase leakage into the medium and MTT test. Pre-treatment of hepatocytes with TCDD and/or PB significantly attenuated SA cytotoxicity, the effects being more pronounced at lower concentrations of SA and shorter periods of incubation. We assumed involvement of CYP1A enzymes in diminution of SA cytotoxicity. Surprisingly, co-treatment with SA and furafylline, an inhibitor of" . "RIV/61989592:15110/06:00003478" . . "165" . "\u0160im\u00E1nek, Vil\u00EDm" . "Putative interactions between quaternary benzo[c]phenanthridine alkaloid sanguinarine (SA) and aryl hydrocarbon receptor/cytochrome P450 CYP1A (AhR/CYP1A) regulatory pathway are the subject of perpetual disputations. The role of CYP1A enzymes and AhR receptor in SA cytotoxicity was anticipated. In this paper, we tested, whether selected inducers of CYP enzymes modulate cytotoxicity of SA in primary cultures of rat hepatocytes. Cells were challenged 48h with dioxin (TCDD; 5nM), phenobarbital (PB; 500microM) or DMSO prior to the treatment with SA. SA itself displayed time- and dose-dependent cytotoxicity as revealed by lactate dehydrogenase leakage into the medium and MTT test. Pre-treatment of hepatocytes with TCDD and/or PB significantly attenuated SA cytotoxicity, the effects being more pronounced at lower concentrations of SA and shorter periods of incubation. We assumed involvement of CYP1A enzymes in diminution of SA cytotoxicity. Surprisingly, co-treatment with SA and furafylline, an inhibitor of"@en . "Mo\u017En\u00E9 interakce mezi kvart\u00E9rn\u00EDm bezofenanthridinov\u00FDm alkaloidem sanguinarinem (SA) a regula\u010Dn\u00ED drahou aryluhlovod\u00EDkov\u00FD receptor/cytochrom P450 CYP1A (AhR/CYP1A) jsou p\u0159edm\u011Btem neutuchaj\u00EDc\u00EDch diskuz\u00ED. P\u0159edpokl\u00E1d\u00E1 se \u00FAloha CYP1A enzym\u016F a AhR receptoru v toxicit\u011B SA. V t\u00E9to pr\u00E1ci jsme testovali, zda vybran\u00E9 induktory CYP enzym\u016F moduluj\u00ED cytotoxicitu SA v prim\u00E1rn\u00EDch kultur\u00E1ch potkan\u00EDch hepatocyt\u016F. Bu\u0148ky byly pre-inkubov\u00E1ny 48 hod s dioxinem (TCDD; 5 nM), fenobarbitalem (PB; 500 mM) nebo DMSO p\u0159ed vlastn\u00ED inkubac\u00ED s SA. Samotn\u00FD SA vykazoval \u010Dasov\u011B a koncentra\u010Dn\u011B z\u00E1vislou cytotoxicitu, monitorov\u00E1no jako aktivita lakt\u00E1t dehydrogenasy v m\u00E9diu a MTT test. Preinkubace hepatocyt\u016F s TCDD nebo PB signifikantn\u011B sn\u00ED\u017Eila cytotoxicitu SA; \u00FA\u010Dinky byly v\u00FDrazn\u011Bj\u0161\u00ED v ni\u017E\u0161\u00EDch koncentrac\u00EDch SA a v krat\u0161\u00EDch period\u00E1ch inkubace. P\u0159edpokl\u00E1dali jsme, \u017Ee zymy CYP1A jsou zapojeny do vymizen\u00ED cytotoxicity SA. Bylo p\u0159ekvapiv\u00E9, \u017Ee inkubace bun\u011Bk se SA spole\u010Dn\u011B s furafylinem, inhibitorem CYP1A enzym\u016F, je\u0161t\u011B v\u00EDce sn\u00ED\u017Eila cytotoxicitu S"@cs . "0378-4274" . . "Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital"@en . "Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital" . "Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital" . "Zda\u0159ilov\u00E1, Ad\u00E9la" . "3" . . "282-288" . . "[76AF4FDA3445]" . . "15110" . . . . "Cytotoxicita sanguinarinu v prim\u00E1rn\u00EDch potkan\u00EDch hepatocytech je sn\u00ED\u017Eena dioxinem a fenobarbitalem"@cs . . "Toxicology Letters" . "\u0160perl\u00EDkov\u00E1, Lucie" . . "Ulrichov\u00E1, Jitka" . . "470277" . "7"^^ . . "IE - Irsko" . "Dvo\u0159\u00E1k, Zden\u011Bk" .