. "Adam, Tom\u00E1\u0161" . "25" . "15110" . . "Fry\u010D\u00E1k, Petr" . "[2B7FC5534CFA]" . "Synthesis and mass spectrometric fragmentation characteristics of imidazole ribosides-analogs of intermediates of purine de novo synthetic pathway" . . "V purinov\u00E9 de novo synt\u00E9ze byly pops\u00E1ny dva d\u011Bdi\u010Dn\u00E9 deficity. Oba tyto enzymov\u00E9 defekty jsou diagnostikov\u00E1ny detekc\u00ED ribosid\u016F - defosforylovan\u00FDch substr\u00E1t\u016F enzym\u016F - v mo\u010Di pacienta. Popisujeme zde synt\u00E9zu a hmotnostn\u011B spektrofotometrickou fragmentaci ribosid\u016F, kter\u00E9 maj\u00ED potenci\u00E1ln\u00ED v\u00FDznam pro diagnostiku defekt\u016F ve druh\u00E9 polovin\u011B dr\u00E1hy. V\u0161echny l\u00E1tky krom\u011B 5-amino-4-imidazolsukcinokarboxamidribosidu byly syntetizov\u00E1ny chemicky z komer\u010Dn\u011B dostupn\u00E9ho 5-amino-4-imidazolkarboxamidribosidu. Fragmenta\u010Dn\u00ED spektra slou\u010Denin byla z\u00EDsk\u00E1na anal\u00FDzou na hmotnostn\u00EDm spektrofotometru s iontovou past\u00ED. B\u011Bhem fragmentace nebylo pozorov\u00E1no otev\u00EDr\u00E1n\u00ED imidazolov\u00E9ho kruhu u \u017E\u00E1dn\u00E9 ze slou\u010Denin. Hlavn\u00ED sm\u011Br fragmentace byl d\u00E1n postupnou ztr\u00E1tou substituent\u016F ve form\u011B mal\u00FDch molekul (NH3, CO2, CO). Fragmentace ribosov\u00E9 \u010D\u00E1sti byla d\u00E1na ztr\u00E1tou molekul vody s n\u00E1sledn\u00FDm rozbit\u00EDm kruhu nebo jeho od\u0161t\u00EDpnut\u00EDm od imidazolov\u00E9ho kruhu jako celku."@cs . "Inherited defects; Metabolism; Purine de novo synthesis"@en . "Vysko\u010Dilov\u00E1, P." . . "RIV/61989592:15110/06:00003197!RIV07-MZ0-15110___" . "Lemr, Karel" . . "502791" . "Two inherited deficiencies have been described in purine de novo synthesis pathway. Both the defects are diagnosed by detecting ribosides-dephosphorylated substrates of the enzymes-in patient's urine. We describe here a synthesis and mass spectrometric fragmentation of ribosides potentially of diagnostic importance for defects in the second part of the pathway. All the species, except 5-amino-4-imidazolesuccinocarboxamideriboside can be synthesized from the commercially available 5-amino-4-imidazolecarboxamideriboside by chemical methods. Fragmentation spectra of the compounds were obtained by the ion trap mass spectrometry. During fragmentation an opening of the imidazole ring was not observed for any of the compounds but loss of its substituents in the form of small molecules (NH3, CO2, CO) is the major route of fragmentation. The ribose moiety cleaves off molecule(s) of water, undergoes a cross-ring cleavage or breaks away as a whole. Copyright (C) Taylor & Francis Group, LLC."@en . . "4"^^ . . "1237-1240" . "Synt\u00E9za a charakteristick\u00E1 hmotnostn\u011B spektrofotometrick\u00E1 fragmentace imidazolov\u00FDch ribosid\u016F - analoga meziprodukt\u016F purinov\u00E9 de novo synt\u00E9zy."@cs . "Horn\u00EDk, P." . "Synthesis and mass spectrometric fragmentation characteristics of imidazole ribosides-analogs of intermediates of purine de novo synthetic pathway"@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . . "6"^^ . . "1525-7770" . . "Nucleosides Nucleotides Nucleic Acids" . . "RIV/61989592:15110/06:00003197" . "Synt\u00E9za a charakteristick\u00E1 hmotnostn\u011B spektrofotometrick\u00E1 fragmentace imidazolov\u00FDch ribosid\u016F - analoga meziprodukt\u016F purinov\u00E9 de novo synt\u00E9zy."@cs . "P(NR7796)" . "11" . "4"^^ . . . . . "Friedeck\u00FD, David" . "Two inherited deficiencies have been described in purine de novo synthesis pathway. Both the defects are diagnosed by detecting ribosides-dephosphorylated substrates of the enzymes-in patient's urine. We describe here a synthesis and mass spectrometric fragmentation of ribosides potentially of diagnostic importance for defects in the second part of the pathway. All the species, except 5-amino-4-imidazolesuccinocarboxamideriboside can be synthesized from the commercially available 5-amino-4-imidazolecarboxamideriboside by chemical methods. Fragmentation spectra of the compounds were obtained by the ion trap mass spectrometry. During fragmentation an opening of the imidazole ring was not observed for any of the compounds but loss of its substituents in the form of small molecules (NH3, CO2, CO) is the major route of fragmentation. The ribose moiety cleaves off molecule(s) of water, undergoes a cross-ring cleavage or breaks away as a whole. Copyright (C) Taylor & Francis Group, LLC." . "Synthesis and mass spectrometric fragmentation characteristics of imidazole ribosides-analogs of intermediates of purine de novo synthetic pathway" . "Synthesis and mass spectrometric fragmentation characteristics of imidazole ribosides-analogs of intermediates of purine de novo synthetic pathway"@en . .