"Cytochrome P450 (CYP) 1A1 attracts attention mainly because of its role in production of carcinogenic reactive metabolites from polycyclic aromatic hydrocarbons such as benzo[a]pyrene, but recent developments indicate its apparent role in cell cycle progression. Expression of the enzyme is subject to regulation by aryl hydrocarbon receptor (AhR). It has been shown that induction of CYP 1A1 in HepG2 cells and primary rat hepatocytes by tetrachloro-p-dibenzodioxin (TCDD) is diminished by colchicine and nocodazole. Both compounds decrease CYP1A1 mRNA, protein, and activity levels in HepG2 cells and mRNA level in primary rat hepatocytes. Neither compound significantly affected [(3)H]-TCDD binding to AhR, thus their effect on AhR transcriptional activity proceeds via indirect means. For colchicine and nocodazole are well-known microtubule interfering agents, we also assessed their effect on microtubule integrity in both cell types under investigation. Both compounds disrupt cytoskeleton integrity with diff" . . "Zapojen\u00ED cytoskeletu do AhR-z\u00E1visl\u00E9 exprese CYP1A1"@cs . "Cytochrom P450 (CYP) 1A1 p\u0159itahuje pozornost hlavn\u011B prosvou \u00FAlohu v tvorb\u011B karcinogenn\u00EDch reaktivn\u00EDch metabolit\u016F z polycyklick\u00FDch aromatick\u00FDch uhlovod\u00EDk\u016F jako je benzo-a-pyren, ale recentn\u00ED poznatky nazna\u010Duj\u00ED jeho zapojen\u00ED do regulace bun\u011B\u010Dn\u00E9ho cyklu. Exprese tohoto enzymu je regulov\u00E1na receptorem pro aromatick\u00E9 uhlovod\u00EDky (AhR). Uk\u00E1zali jsme, \u017Ee indukce CYP1A1 dioxinem v prim\u00E1rn\u00EDch potkan\u00EDch hepatocytech a HepG2 bu\u0148k\u00E1ch je naru\u0161ena kolchicinem a nokodazolem. Ob\u011B slou\u010Deniny sn\u00ED\u017Eily obsah CYP1A1 proteinu, mRNA a katalytickou aktivitu v HepG2 bu\u0148k\u00E1ch a prim\u00E1rn\u00EDch potkan\u00EDch hepatocytech. \u017D\u00E1dn\u00E1 ze slou\u010Denin neovlivnila vazbu 3H-TCDD do AhR, tedy jejich \u00FA\u010Dinek na transkrip\u010Dn\u00ED aktivitu AhR je nep\u0159\u00EDm\u00FD. Jeliko\u017E kolchicin a nokodazol jsou l\u00E1tky naru\u0161uj\u00EDc\u00ED mikrotubuly, zkoumali jsme jejich \u00FA\u010Dinek na integritu mikrotubul\u016F v obou typech bun\u011Bk.Ob\u011B slou\u010Deniny rozbily mikrotubul\u00E1rn\u00ED s\u00ED\u0165 s odli\u0161nou \u00FA\u010Dinnost\u00ED, v z\u00E1vislosti na typu bun\u011Bk. Pozorovan\u00E9 potla\u010Den\u00ED transkrip\u010Dn\u00ED aktivity AhR kolchicinem a nokodazolem by v Hep"@cs . "7" . . . "Current Drug Metabolism" . "13"^^ . "Involvement of cytoskeleton in AhR-dependent CYP1A1 expression" . . . "Zapojen\u00ED cytoskeletu do AhR-z\u00E1visl\u00E9 exprese CYP1A1"@cs . . "AhR receptor; Cell Cycle; Cytochrome P450; Drug Metabolism; Microtubules"@en . . "480383" . "3" . "Maurel, Patrick" . "Cytochrome P450 (CYP) 1A1 attracts attention mainly because of its role in production of carcinogenic reactive metabolites from polycyclic aromatic hydrocarbons such as benzo[a]pyrene, but recent developments indicate its apparent role in cell cycle progression. Expression of the enzyme is subject to regulation by aryl hydrocarbon receptor (AhR). It has been shown that induction of CYP 1A1 in HepG2 cells and primary rat hepatocytes by tetrachloro-p-dibenzodioxin (TCDD) is diminished by colchicine and nocodazole. Both compounds decrease CYP1A1 mRNA, protein, and activity levels in HepG2 cells and mRNA level in primary rat hepatocytes. Neither compound significantly affected [(3)H]-TCDD binding to AhR, thus their effect on AhR transcriptional activity proceeds via indirect means. For colchicine and nocodazole are well-known microtubule interfering agents, we also assessed their effect on microtubule integrity in both cell types under investigation. Both compounds disrupt cytoskeleton integrity with diff"@en . . . . "4"^^ . . "Pascussi, Jean-Marc" . . "P(1P05ME767), P(GP303/04/P074), Z(MSM6198959216)" . "Involvement of cytoskeleton in AhR-dependent CYP1A1 expression" . . . "1389-2002" . . "15110" . . . . "RIV/61989592:15110/06:00003106" . "Involvement of cytoskeleton in AhR-dependent CYP1A1 expression"@en . "6"^^ . "NL - Nizozemsko" . "301-313" . . "Involvement of cytoskeleton in AhR-dependent CYP1A1 expression"@en . "Modriansk\u00FD, Martin" . . . . "[E360CD985C8D]" . "Vrzal, Radim" . "Dvo\u0159\u00E1k, Zden\u011Bk" . "RIV/61989592:15110/06:00003106!RIV07-GA0-15110___" . "Ulrichov\u00E1, Jitka" . .