. . . "CZ - \u010Cesk\u00E1 republika" . "Kosina, Pavel" . "Posouzen\u00ED bezpe\u010Dnosti sanguiritrinu, frakce alkaloid\u016F z Macleaya cordata, na potkanech"@cs . "Lichnovsk\u00FD, V\u00E1clav" . . "4" . . . "[99FB71FC5A30]" . . "Svobodov\u00E1, Alena" . "Zda\u0159ilov\u00E1, Ad\u00E9la" . "P(GP203/03/D237), Z(MSM6198959216)" . "Jirovsk\u00FD, David" . . "Bezpe\u010Dnost subchronick\u00E9 d\u00E1vky sanguiritrinu, sm\u011Bsi kvartern\u00EDch benzo[c]fenanthridinov\u00FDch alkaloid\u016F (KBA) sanguinarinu (SA) a chelerythrinu (CHE), izolovan\u00E9 z Macleaya cordata byla studov\u00E1na na potkanech. Potkani byli krmeni dietou obsahuj\u00EDc\u00ED 120 ppm sanguiritrinu (100 ppm KBA) po dobu 109 dn\u00ED. Pr\u016Fb\u011B\u017En\u011B byla sledov\u00E1na spot\u0159eba krmiva a hmotnost zv\u00ED\u0159at. Obsah KBA ve vybran\u00FDch tk\u00E1n\u00EDch a v plasm\u011B byl stanoven HPLC. P\u0159ibli\u017En\u011B 2 % KBA byly absorbov\u00E1ny v GIT a 98 % bylo vylou\u010Deno trusem. Hladina bilirubinu, mo\u010Doviny, kreatininu, glomerul\u00E1rn\u00ED filtrace, AST, ALT, GMT, ALP a celkov\u00E1 antioxida\u010Dn\u00ED kapacita byly stanoveny v plasm\u011B. V j\u00E1trech bylo zm\u011B\u0159eno mno\u017Estv\u00ED GSH, lipoperoxida\u010Dn\u00EDch produkt\u016F, aktivita SOD a GPx a celkov\u00E9 mno\u017Estv\u00ED cytochromu P450. Po\u0161kozen\u00ED jadern\u00E9 DNA bylo sledov\u00E1no; metodou 32P-postlabelingu nebyly detekov\u00E1ny DNA-adukty v jatern\u00ED jadern\u00E9 ani v mitochondri\u00E1ln\u00ED DNA. Na potkanech nebyly pozorov\u00E1ny \u017E\u00E1dn\u00E9 ne\u017E\u00E1douc\u00ED zm\u011Bny. KBA nezp\u016Fsobily zm\u011Bny na epitelu st\u0159eva, jatern\u00ED tk\u00E1ni a neovlivnily \u017E\u00E1dn\u00FD z"@cs . . . . . . "145-155" . "Posouzen\u00ED bezpe\u010Dnosti sanguiritrinu, frakce alkaloid\u016F z Macleaya cordata, na potkanech"@cs . "Safety assessment of sanguiritrin, alkaloid fraction of Macleya cordata, in rats"@en . . . . "12"^^ . "13"^^ . . "15110" . . . "Stiborov\u00E1, Marie" . "Anzenbacherov\u00E1, Eva" . "51" . "Safety assessment of sanguiritrin, alkaloid fraction of Macleya cordata, in rats" . . "Safety assessment of sanguiritrin, alkaloid fraction of Macleya cordata, in rats"@en . "RIV/61989592:15110/06:00003103!RIV07-GA0-15110___" . . "11"^^ . "Benzo[c]phenanthridine alkaloids; Oral administration; Biochemical markers; Oxidative stress; DNA damage; Cytochrome P450"@en . "Psotov\u00E1, Jitka" . . "\u0160im\u00E1nek, Vil\u00EDm" . . . . . "Safety assessment of sanguiritrin, alkaloid fraction of Macleya cordata, in rats" . "The subchronic safety of sanguiritrin, a mixture of sanguinarine (SA) and chelerythrine (CHE) quaternary benzo[c]phenanthridine alkaloids (QBA), obtained from Macleaya cordata was assessed. Rats were fed a diet containing 120 ppm sanguiritrin (100 ppm QBA) for 109 days. The feed consumption and the animal weight were monitored. The content of QBA in selected tissues and plasma was determined using HPLC. It was evidenced that 2 % of QBA were absorbed through the GIT while 98 % were excreted in the feces. In plasma, bilirubin, urea, creatinine, glomerular filtration, AST, ALT, GMT, ALP and total antioxidant capacity were determined. In liver, GSH level, lipoperoxidation products, SOD and GPx activities and total amount of cytochrome P450 were evaluated. Damage to nuclear DNA was assessed; a 32P-postlabeling assay proved that no DNA-adducts were detected in nuclear and mitochrondrial DNA in liver. No adverse effects were observed on rat organism. QBA had no influence on the gut mucosal epithelium, liver"@en . "Hrb\u00E1\u010D, Jan" . . . "498354" . "Ve\u010De\u0159a, Rostislav" . "The subchronic safety of sanguiritrin, a mixture of sanguinarine (SA) and chelerythrine (CHE) quaternary benzo[c]phenanthridine alkaloids (QBA), obtained from Macleaya cordata was assessed. Rats were fed a diet containing 120 ppm sanguiritrin (100 ppm QBA) for 109 days. The feed consumption and the animal weight were monitored. The content of QBA in selected tissues and plasma was determined using HPLC. It was evidenced that 2 % of QBA were absorbed through the GIT while 98 % were excreted in the feces. In plasma, bilirubin, urea, creatinine, glomerular filtration, AST, ALT, GMT, ALP and total antioxidant capacity were determined. In liver, GSH level, lipoperoxidation products, SOD and GPx activities and total amount of cytochrome P450 were evaluated. Damage to nuclear DNA was assessed; a 32P-postlabeling assay proved that no DNA-adducts were detected in nuclear and mitochrondrial DNA in liver. No adverse effects were observed on rat organism. QBA had no influence on the gut mucosal epithelium, liver" . "Veterin\u00E1rn\u00ED medic\u00EDna" . "0375-8427" . "Vi\u010Dar, Jaroslav" . . "Ulrichov\u00E1, Jitka" . . "RIV/61989592:15110/06:00003103" . .