. "Cryopreservation oVers the potential to maximize the use and availability of biological materials that have a limited supply. This study demonstrates an enhanced technique for the parallel cryopreservation of a series of liver tissue slices using a tray modeled from aluminium foil and low concentrations of a cryoprotectant. Cooling and warming rates of approximately 2000 and 3900 \u00B0Cmin\u02C71, respectively, were achieved as the thermal capacity of the foil-tray was significantly reduced compared to the aluminium sandwich device introduced by Day et al. [S.H. Day, D.A. Nicoll-Grifith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Additionally, the two critical steps involved in the sandwich approach, i.e., clamping the plates and complete filling of the entire space between the plates with liquid, can be omitted using the foil tray. The viability of the slices was verified by measuring tetrazolium salt reduction capacity, cytosolic enzyme lact" . "Cryobiology" . . . . "Florschutz, Anthony V." . . "0011-2240" . "P(OC B17.20), Z(MSM6198959205)" . . "A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices" . . "Kryoprezervace poskytuje mo\u017Enost maximalizovat vyu\u017Eit\u00ED a dostupnost biologick\u00FDch materi\u00E1l\u016F, jejich\u017E zdroje jsou omezen\u00E9. Tato studie p\u0159edkl\u00E1d\u00E1 zlep\u0161enou techniku sou\u010Dasn\u00E9 kryoprezervace serie \u0159ez\u016F jatern\u00ED tk\u00E1n\u011B s vyu\u017Eit\u00EDm p\u0159\u00EDpravku z hlin\u00EDkov\u00E9 folie p\u0159i n\u00EDzk\u00E9 koncentraci kryoprotektantu. Rychlost zchlazen\u00ED a zah\u0159\u00EDv\u00E1n\u00ED p\u0159ibli\u017En\u011B 2000 a 3900 \u00B0C.min-1 je dosa\u017Eena v\u00FDznamn\u00FDm sn\u00ED\u017Een\u00EDm tepeln\u00E9 kapacity hlin\u00EDkov\u00E9 folie ve srovn\u00E1n\u00ED se sandwichov\u00FDm za\u0159\u00EDzen\u00EDm, popsan\u00FDm Dayem et al. [S.H. Day, D.A. Nicoll-Griffith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Viabilita \u0159ez\u016F byla ov\u011B\u0159ov\u00E1na m\u011B\u0159en\u00EDm redukce tetrazoliov\u00E9 soli, uvol\u0148ov\u00E1n\u00ED cytosolov\u00E9 lakt\u00E1tdehydrogen\u00E1zy do media a metabolismu ethoxykumarinu."@cs . "\u010Cervenkov\u00E1, Kate\u0159ina" . "Uherkov\u00E1, Lenka" . . "2" . "52" . "A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices" . . . . . . . "Nov\u00E1 zjednodu\u0161en\u00E1 technika ultrarychl\u00E9ho zmrazov\u00E1n\u00ED pro kryoprezervaci tk\u00E1\u0148ov\u00FDch \u0159ez\u016F"@cs . "463736" . . "Nov\u00E1 zjednodu\u0161en\u00E1 technika ultrarychl\u00E9ho zmrazov\u00E1n\u00ED pro kryoprezervaci tk\u00E1\u0148ov\u00FDch \u0159ez\u016F"@cs . . . "RIV/61989592:15110/06:00002714" . "Rypka, Miroslav" . "A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices"@en . . "[771D980F8CE5]" . "193-199" . . . "A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices"@en . . "Cryopreservation oVers the potential to maximize the use and availability of biological materials that have a limited supply. This study demonstrates an enhanced technique for the parallel cryopreservation of a series of liver tissue slices using a tray modeled from aluminium foil and low concentrations of a cryoprotectant. Cooling and warming rates of approximately 2000 and 3900 \u00B0Cmin\u02C71, respectively, were achieved as the thermal capacity of the foil-tray was significantly reduced compared to the aluminium sandwich device introduced by Day et al. [S.H. Day, D.A. Nicoll-Grifith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Additionally, the two critical steps involved in the sandwich approach, i.e., clamping the plates and complete filling of the entire space between the plates with liquid, can be omitted using the foil tray. The viability of the slices was verified by measuring tetrazolium salt reduction capacity, cytosolic enzyme lact"@en . "15110" . "5"^^ . "Vesel\u00FD, Jaroslav" . . "7"^^ . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "RIV/61989592:15110/06:00002714!RIV07-MSM-15110___" . . "Cryopreservation; Precision-cut slices; Ultra-rapid freezing; Warming rate; Mouse liver"@en . "Poczatkov\u00E1, Hana" . "6"^^ .