. . "In vitro testov\u00E1n\u00ED kostn\u00EDho cementu obohacen\u00E9ho gentamicinem a vankomycinem v prevenci periprotetick\u00FDch infekc\u00ED"@cs . "6"^^ . . . . "In vitro testing of gentamicin-vancomycin loaded bone cement to prevent prosthetic joint infection" . "In vitro testing of gentamicin-vancomycin loaded bone cement to prevent prosthetic joint infection"@en . . "Prosthetic joint infection; Polyethylenacrylate; Antibiotic loaded cement; Vancomycin; Gentamicin; Staphylococcus aureus; Staphylococcus epidermidis"@en . . "Sepsis is a greatly feared complication of total joint arthtoplasty. One key question is how to prevent perioperative bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro effects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh molecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identified using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantification. Materials without antibioti"@en . . "Pant\u016F\u010Dek, Roman" . . "RIV/61989592:15110/05:00002013" . "Kesselov\u00E1, Michaela" . . "CZ - \u010Cesk\u00E1 republika" . "[EC2B7C567D9F]" . "P(NM7680)" . . "6"^^ . "153-158" . "Sepsis is a greatly feared complication of total joint arthtoplasty. One key question is how to prevent perioperative bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro effects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh molecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identified using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantification. Materials without antibioti" . . . "15110" . . "Biomedical Papers" . . . "Infekce kloubn\u00EDch n\u00E1hrad je ob\u00E1vanou komplikac\u00ED endoprotetiky. Jednou z kl\u00ED\u010Dov\u00FDch ot\u00E1zek prevence je, jak zabr\u00E1nit adhezi bakteri\u00ED na povrch implant\u00E1tu. C\u00EDlem pr\u00E1ce bylo testovat in vitro preventivn\u00ED potenci\u00E1l cementu obohacen\u00E9ho n\u00EDzkou d\u00E1vkou antibiotika (vankomycinu a gentamicinu). Bakteri\u00E1ln\u00ED z\u00E1t\u011B\u017E p\u0159edstavovali r\u016Fzn\u00E9 koncentrace v nemocnici z\u00EDskan\u00FDch stafylokok\u016F, kter\u00E9 byly charakterizov\u00E1ny kultiva\u010Dn\u011B, chemicky i geneticky. Kontroln\u00ED skupiny tvo\u0159ily cementy bez antibiotika, firemn\u011B vyr\u00E1b\u011Bn\u00E9 cementy s antibiotikem a polyetyl\u00E9nov\u00E9 povrchy. Bakteri\u00E1ln\u00ED kolonizace byla hodnocena jednak kultiva\u010Dn\u011B, jednak semikvantitativn\u011B za pomoci REM. Krom\u011B testovan\u00E9ho cementu s kombinac\u00ED vancomycin-gentamicin nebyl \u017E\u00E1dn\u00FD dal\u0161\u00ED materi\u00E1l schopen konzistentn\u011B br\u00E1nit adhezi pou\u017Eit\u00FDch bakteri\u00ED. Z toho vyvozujeme, \u017Ee by bylo mo\u017En\u00E9 tuto kombinaci vyu\u017E\u00EDvat klinicky u pacient\u016F se zv\u00FD\u0161enou predispozic\u00ED ke vzniku infekce kloubn\u00ED n\u00E1hrady."@cs . "In vitro testov\u00E1n\u00ED kostn\u00EDho cementu obohacen\u00E9ho gentamicinem a vankomycinem v prevenci periprotetick\u00FDch infekc\u00ED"@cs . "Gallo, Ji\u0159\u00ED" . . "Kol\u00E1\u0159, Milan" . "RIV/61989592:15110/05:00002013!RIV06-MZ0-15110___" . "149" . . "In vitro testing of gentamicin-vancomycin loaded bone cement to prevent prosthetic joint infection" . . "4"^^ . "In vitro testing of gentamicin-vancomycin loaded bone cement to prevent prosthetic joint infection"@en . "Floesch\u00FCtz, Anthony Vatroslav" . "1213-8118" . "1" . . . . "Novotn\u00FD, Radko" . "524485" .