. "2" . "Zm\u011Bny v hladin\u00E1ch mRNA intracelul\u00E1rn\u00EDch regul\u00E1tor\u016F mastn\u00FDch kyselin p\u016Fsoben\u00EDm neesterifikovan\u00FDch mastn\u00FDch kyselin a dehydroepiandrosteronu na lidsk\u00E9 hepatomov\u00E9 bu\u0148ky HepG2"@cs . . "[3F66F2ED46DC]" . "RIV/61989592:15110/05:00001984!RIV07-MSM-15110___" . "Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone" . . . . . . "Zm\u011Bny v hladin\u00E1ch mRNA intracelul\u00E1rn\u00EDch regul\u00E1tor\u016F mastn\u00FDch kyselin p\u016Fsoben\u00EDm neesterifikovan\u00FDch mastn\u00FDch kyselin a dehydroepiandrosteronu na lidsk\u00E9 hepatomov\u00E9 bu\u0148ky HepG2"@cs . "6"^^ . . "Uherkov\u00E1, Lenka" . "251-256" . "Byl sledov\u00E1n vliv neesterifikovan\u00FDch mastn\u00FDch kyselin (NEFA) a hormonu dehydroepiandrosteronu na hladiny mRNA isoforem δ a  proteinkin\u00E1zy C (PKC) a mRNA liver fatty acid binding proteinu (L-FABP) v lidsk\u00FDch hepatomov\u00FDch bu\u0148k\u00E1ch HepG2. Bu\u0148ky byly po dobu experimentu udr\u017Eov\u00E1ny v mediu s n\u00EDzk\u00FDm obsahem s\u00E9ra a albuminu. Zvolen\u00E1 koncentrace mastn\u00FDch kyselin byla 100 \u00B5M a \u010Dasov\u00E9 intervaly 4 a 20 hodin. Za t\u011Bchto podm\u00EDnek nasycen\u00E9 (palmitov\u00E1, stearov\u00E1) a mononenasycen\u00E9 (olejov\u00E1) kyseliny pom\u011Brn\u011B selektivn\u011B stimulovaly mRNA PKC . Neo\u010Dek\u00E1van\u011B jsme zjistili, \u017Ee tyto mastn\u00E9 kyseliny tak\u00E9 sni\u017Euj\u00ED hladinu mRNA L-FABP. DHEA ve farmakologick\u00FDch d\u00E1vk\u00E1ch (100 μM) vyvol\u00E1v\u00E1 znateln\u00E9 zv\u00FD\u0161en\u00ED hladin mRNA PKC-δ a -ε. I kdy\u017E v tomto p\u0159\u00EDsp\u011Bvku nebyly zkoum\u00E1ny molekul\u00E1rn\u00ED mechanismy na pozad\u00ED zji\u0161t\u011Bn\u00FDch zm\u011Bn, na\u0161e v\u00FDsledky zd\u016Fraz\u0148uj\u00ED, \u017Ee modulace hladin mRNA, indukovan\u00E9 NEFA, p\u0159edstavuj\u00ED dal\u0161\u00ED mechanismus, kter\u00FDm mohou NEFA v prost\u0159ed\u00ED ovliv\u0148ovat metabolickou homeo"@cs . . "\u010Cervenkov\u00E1, Kate\u0159ina" . "Vesel\u00FD, Jaroslav" . . "The effects of non-esterified fatty acids (NEFA) and hormone dehydroepiandrosterone (DHEA) on the levels of mRNAs of protein kinase C (PKC) -δ and -ε isoforms and those of liver fatty acid binding protein (L-FABP) were investigated in the human hepatoma HepG2 cell line. The cells were kept in low-serum, low-albumin medium during experiments. Low FA levels (100 μM) and time intervals of 4 h and 20 h were used. In these conditions, the saturated (palmitic, stearic) and monounsaturated (oleic) acids rather selectively stimulated PKC-ε mRNA levels. Unexpectedly, we found that these acids also suppressed liver fatty-acid binding protein (L-FABP) mRNA levels. DHEA in pharmacological doses (100 μM) produced a significant increase in PKC-δ and -ε mRNA levels. Although molecular mechanisms underlying the identified changes have not been investigated in this paper, our findings emphasize that NEFA-induced modulation of mRNA levels of key signalling" . . "Biomedical Papers" . "Rypka, Miroslav" . . "P(1P05OC063), P(1P05OC064), P(OC B17.20), Z(MSM6198959205)" . . . . . "Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone"@en . . "The effects of non-esterified fatty acids (NEFA) and hormone dehydroepiandrosterone (DHEA) on the levels of mRNAs of protein kinase C (PKC) -δ and -ε isoforms and those of liver fatty acid binding protein (L-FABP) were investigated in the human hepatoma HepG2 cell line. The cells were kept in low-serum, low-albumin medium during experiments. Low FA levels (100 μM) and time intervals of 4 h and 20 h were used. In these conditions, the saturated (palmitic, stearic) and monounsaturated (oleic) acids rather selectively stimulated PKC-ε mRNA levels. Unexpectedly, we found that these acids also suppressed liver fatty-acid binding protein (L-FABP) mRNA levels. DHEA in pharmacological doses (100 μM) produced a significant increase in PKC-δ and -ε mRNA levels. Although molecular mechanisms underlying the identified changes have not been investigated in this paper, our findings emphasize that NEFA-induced modulation of mRNA levels of key signalling"@en . "Poczatkov\u00E1, Hana" . . . "149" . . . "RIV/61989592:15110/05:00001984" . . "5"^^ . "CZ - \u010Cesk\u00E1 republika" . "1213-8118" . "Fatty acids; Protein kinase C (PKC); Liver fatty acid binding protein (L-FABP); Insulin resistance; HepG2 cells"@en . "6"^^ . "15110" . "Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone"@en . "Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone" . . "514913" . . "Bogdanov\u00E1, Kate\u0159ina" . .