"Kol\u00E1\u0159, Zden\u011Bk" . "15110" . "6"^^ . . "Comparison of Protective Effect of Protein and DNA Vaccines hsp90 in Murine Model of Systemic Candidiasis" . "ABSTRACT. Preventive vaccination by a hsp90-expressing DNA vaccine and recombinant hsp90 proteinvaccine, both derived from the Candida albicans hsp90 using BALB-c mouse model of systemic candidiasis,was performed. Hsp90 mRNA was cloned from a clinical isolate of C. albicans, converted to cDNA andcloned into vaccination plasmid pVAX1. Two methods of DNA application were tested: intramuscular (i.m.)and intradermal (i.d.) injection. Recombinant protein was applied by i.d. injection with Freund's adjuvant;the control groups received PBS or Freund's adjuvant only. Mice were vaccinated and after 19 d re-vaccinated.After 3 weeks, the mice were challenged with the live C. albicans in a dose of 5 x 106 CFU per mouse.After the challenge, the mice vaccinated i.d. with DNA vaccine survived for 39 and 64 % longer comparedto those receiving Freund's adjuvant and/or PBS, respectively. The i.m. application of the DNA vaccine didnot provide any significant protectivity. The serum level of anti-candida-h" . "Comparison of Protective Effect of Protein and DNA Vaccines hsp90 in Murine Model of Systemic Candidiasis"@en . . "Z(MSM 151100002)" . "Byla provedena preventivn\u00ED vakcinace experiment\u00E1ln\u00EDch BALB/c my\u0161\u00ED DNA vakc\u00EDnou exprimuj\u00EDc\u00ED hsp90 protein z Candida albicans a vakcinace t\u00EDmto proteinem p\u0159ipraven\u00FDm rekombinantn\u00ED technologi\u00ED. Efektivita byla testov\u00E1na na modelu syst\u00E9mov\u00E9 kandid\u00F3zy. Z mikrobiologicky ov\u011B\u0159en\u00E9ho kmene C. albicans byla izolov\u00E1na mRNA hsp90, p\u0159eps\u00E1na do cDNA a klonov\u00E1na do vakcina\u010Dn\u00EDho plasmidu pVAX1. Byly pou\u017Eity dv\u011B metody aplikace DNA: intramuskul\u00E1rn\u00ED (i.m.) nebo intraderm\u00E1ln\u00ED (i.d.) injekce. Rekombinantn\u00ED protein byl injikov\u00E1n ve Freundov\u011B adjuvans i.d.; kontroln\u00ED skupin\u011B byl aplikov\u00E1n PBS nebo Freundovo adjuvans. My\u0161i byly dvakr\u00E1t vakcinov\u00E1ny s odstupem 19 dn\u00ED. T\u0159i t\u00FDdny po druh\u00E9 d\u00E1vce byly my\u0161i i.v. infikov\u00E1ny 5x10exp6 CFU \u017Eiv\u00FDch C. albicans. I.d. DNA vakcinace prodlou\u017Eila p\u0159e\u017E\u00EDv\u00E1n\u00ED my\u0161\u00ED v\u016F\u010Di kontroln\u00ED skupin\u011B s Freundov\u00FDm adjuvans o 39% a v\u016F\u010Di skupin\u011B s PBS o 64%. I.m. DNA vakcinace nebyla spojena s pr\u016Fkazem protektivity. S\u00E9rov\u00E9 hladiny IgG imunoglobulin\u016F specificky reaguj\u00EDc\u00EDch s hsp90 C. albicans korelovaly s p\u0159e\u017E\u00EDv"@cs . "Comparison of Protective Effect of Protein and DNA Vaccines hsp90 in Murine Model of Systemic Candidiasis" . "77-82" . . . "ABSTRACT. Preventive vaccination by a hsp90-expressing DNA vaccine and recombinant hsp90 proteinvaccine, both derived from the Candida albicans hsp90 using BALB-c mouse model of systemic candidiasis,was performed. Hsp90 mRNA was cloned from a clinical isolate of C. albicans, converted to cDNA andcloned into vaccination plasmid pVAX1. Two methods of DNA application were tested: intramuscular (i.m.)and intradermal (i.d.) injection. Recombinant protein was applied by i.d. injection with Freund's adjuvant;the control groups received PBS or Freund's adjuvant only. Mice were vaccinated and after 19 d re-vaccinated.After 3 weeks, the mice were challenged with the live C. albicans in a dose of 5 x 106 CFU per mouse.After the challenge, the mice vaccinated i.d. with DNA vaccine survived for 39 and 64 % longer comparedto those receiving Freund's adjuvant and/or PBS, respectively. The i.m. application of the DNA vaccine didnot provide any significant protectivity. The serum level of anti-candida-h"@en . . "RIV/61989592:15110/05:00001983" . . "RIV/61989592:15110/05:00001983!RIV06-MSM-15110___" . . "R\u016F\u017Ei\u010Dkov\u00E1, Katharine" . "5"^^ . . . "[9F415DA46A05]" . "Wudattu, Nalini K." . "1" . . "8"^^ . "Srovn\u00E1n\u00ED protektivn\u00EDho efektu proteinov\u00E9 a DNA vakc\u00EDn hsp90 na modelu syst\u00E9mov\u00E9 kandid\u00F3zy experiment\u00E1ln\u00EDch my\u0161\u00ED."@cs . . "Comparison of Protective Effect of Protein and DNA Vaccines hsp90 in Murine Model of Systemic Candidiasis"@en . . "Srovn\u00E1n\u00ED protektivn\u00EDho efektu proteinov\u00E9 a DNA vakc\u00EDn hsp90 na modelu syst\u00E9mov\u00E9 kandid\u00F3zy experiment\u00E1ln\u00EDch my\u0161\u00ED."@cs . "Kafkov\u00E1, Leona" . . . "Ra\u0161ka, Milan" . "0015-5632" . . . . "515903" . "CZ - \u010Cesk\u00E1 republika" . "\u0160ebestov\u00E1, Martina" . "DNA; vaccine; hsp90; candida"@en . . "B\u011Bl\u00E1kov\u00E1, Jana" . "Weigl, Ev\u017Een" . "Folia Microbiologica" . . "50" . .