. "Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand capacity for aro"@en . . "1100" . . "Batch immunoextraction method for an effective purification of aromatic cytokinins."@en . "Popa, Igor" . . . "Hauserov\u00E1, Eva" . "Strnad, Miroslav" . "8"^^ . . "Journal of Chromatography A: Symposium Volumes" . "antibody; batch immunoextraction; 6-benzylaminopurine"@en . . . "[C31E3776BAE1]" . "Hajd\u00FAch, Mari\u00E1n" . "9"^^ . . "0021-9673" . "116-125" . . . "RIV/61989592:15110/05:00001951" . "NL - Nizozemsko" . . "513575" . "Vydra, David" . "Batch immunoextraction method for an effective purification of aromatic cytokinins." . . "1" . "RIV/61989592:15110/05:00001951!RIV06-MSM-15110___" . "Batch immunoextraction method for an effective purification of aromatic cytokinins."@en . "P\u0159irozen\u00E9 benzylaminopuriny p\u0159itomn\u00E9 v rostlin\u00E1ch maj\u00ED vysokou bilogickou aktivitu. Dal\u0161\u00ED byly syntetizovan\u00E9 jak np\u0159. 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), kter\u00E9 vykazuj\u00ED vysokou protin\u00E1dorovou aktivitu v in vitro podm\u00EDnk\u00E1ch."@cs . "Swaczynov\u00E1, Jana" . "Dole\u017Eal, Karel" . . "S\u00E9rie imunoextrak\u010Dn\u00EDch metod na efektivn\u00ED purifikaci aromatick\u00FDch cytokinin\u016F."@cs . . . . . "S\u00E9rie imunoextrak\u010Dn\u00EDch metod na efektivn\u00ED purifikaci aromatick\u00FDch cytokinin\u016F."@cs . "Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand capacity for aro" . "10"^^ . . "Lenobel, Ren\u00E9" . . "15110" . "Batch immunoextraction method for an effective purification of aromatic cytokinins." . . "Fuksov\u00E1, Kv\u011Btoslava" . . "Z(MSM6198959216)" .