"N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules."@en . . "576255" . "2"^^ . . . "N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules." . "141-144" . . . "Biomedical Papers" . "V t\u00E9to pr\u00E1ci jsme studovali, zda jsou mikrotubuly a %22heat shock%22 protein 90 (Hsp90) v bu\u0148k\u00E1ch HL-60 diferencovan\u00FDch dimethylsulfoxidem zapojeny do oxidativn\u00EDho vzplanut\u00ED aktivovan\u00E9ho 12-O-myristoylforbol-13-acet\u00E1tem (PMA) a peptidem N-formyl-Met-Leu-Phe (fMLP). V\u00FDsledky uk\u00E1zaly, \u017Ee l\u00E1tky interaguj\u00EDc\u00ED s mikrotubuly, paklitaxel (1-5 #M), kolchicin (1-100 #M), nokodazol (1-20 #M) a vinkristin (1-50 #M), neovliv\u0148uj\u00ED oxidativn\u00ED vzplanut\u00ED aktivovan\u00E9 PMA ani fMLP. Naproti tomu radicikol, inhibitor proteinu Hsp90, inhiboval \u010Dasov\u011B a koncentra\u010Dn\u011B z\u00E1visl\u00FDm zp\u016Fsobem oxidativn\u00ED vzplanut\u00ED aktivovan\u00E9 fMLP, p\u0159i\u010Dem\u017E hodnota IC50 m\u011Bla p\u0159i preinkubaci 30 min hodnotu 16,5 # 3,5 #M. Z v\u00FDsledk\u016F usuzujeme, \u017Ee oxidativn\u00ED vzplanut\u00ED v diferencovan\u00FDch bu\u0148k\u00E1ch HL-60 aktivovan\u00E9 PMA i fMLP je nez\u00E1visl\u00E9 na mikrotubulech a \u017Ee vzplanut\u00ED vyvolan\u00E9 fMLP vy\u017Eaduje aktivitu Hsp90."@cs . "Modriansk\u00FD, Martin" . "N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules."@en . "4"^^ . . . . . "N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules." . "Radicicol; Hsp90; Microtubules; Oxidative burst; HL-60"@en . . . "Oxidativn\u00ED vzplanut\u00ED aktivovan\u00E9 peptidem N-formyl-Met-Leu-Phe vy\u017Eaduje v bu\u0148k\u00E1ch HL-60 diferencovan\u00FDch dimethylsulfoxidem aktivn\u00ED Hsp90, ale nevy\u017Eaduje neporu\u0161en\u00E9 mikrotubuly"@cs . "In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 microM), colchicine (1-100 microM), nocodazole (1-20 microM), and vincristine (1-50 microM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 +/- 3.5 microM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity."@en . . "[F54B72B9E76C]" . . "In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 microM), colchicine (1-100 microM), nocodazole (1-20 microM), and vincristine (1-50 microM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 +/- 3.5 microM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity." . . "148" . "15110" . "2" . . . . "Z(MSM 151100003)" . "RIV/61989592:15110/04:00002001!RIV06-MSM-15110___" . "Oxidativn\u00ED vzplanut\u00ED aktivovan\u00E9 peptidem N-formyl-Met-Leu-Phe vy\u017Eaduje v bu\u0148k\u00E1ch HL-60 diferencovan\u00FDch dimethylsulfoxidem aktivn\u00ED Hsp90, ale nevy\u017Eaduje neporu\u0161en\u00E9 mikrotubuly"@cs . "Vrba, Ji\u0159\u00ED" . "CZ - \u010Cesk\u00E1 republika" . "1213-8118" . "RIV/61989592:15110/04:00002001" . . "2"^^ .