. . . "[2FF22CB84956]" . "RIV/61989592:15110/04:00000899" . "Cytochromes P450;CYP2E1;CYP3A4;haematopoietic stem cells;progenitor cells"@en . . "Pomoc\u00ED Westernblotu byla prok\u00E1z\u00E1na p\u0159\u00EDtomnost CYP2E1 v lidsk\u00FDch CD34+ bu\u0148k\u00E1ch (hematopoietick\u00E9 kmenov\u00E9 a progenitorov\u00E9 bu\u0148ky separovan\u00E9 z perifern\u00ED krve). Poprv\u00E9 byla tak\u00E9 detekov\u00E1na specifick\u00E1 enzymov\u00E1 aktivita cytochromu P4502E1 (hydroxylace chlorzoxazonu). Nebyl nalezen \u017E\u00E1dn\u00FD CYP3A4 protein ani nebyla zji\u0161t\u011Bna oxidace nifedipinu, specifick\u00E9ho substr\u00E1tu pro tento enzym. Z\u00EDskan\u00E9 v\u00FDsledky ukazuj\u00ED odli\u0161nosti v obsahu aktivn\u00EDch forem cytochrom\u016F P450 v populaci kmenov\u00FDch a progenitorov\u00FDch bun\u011Bk z\u00EDskan\u00FDch z r\u016Fzn\u00FDch species, proto\u017Ee nap\u0159. bylo prok\u00E1z\u00E1no (Avital et al. 2001), \u017Ee CYP3A2 (potkan\u00ED forma podobn\u00E1 lidsk\u00E9mu CYP3A4) je exprimov\u00E1na v bu\u0148k\u00E1ch z\u00EDskan\u00FDch z kostn\u00ED d\u0159en\u011B potkana."@cs . . "P(NL7295)" . . "P\u0159\u00EDtomnost cytochrom\u016F P450 v lidsk\u00FDch CD34(+) hematopoetick\u00FDch progenitorov\u00FDch bu\u0148k\u00E1ch"@cs . "2"^^ . "Human CD34(+) cells (haematopoietic stem and progenitor cells separated from peripheral blood) were shown to express CYP2E1 protein by Western blotting. For the first time, the specific CYP2E1 activity (chlorzoxazone 6-hydroxylation) was also detected. No CYP3A4 protein neither the CYP3A-specific nifedipine oxidase activity were detectable. The results obtained indicate differences in content of active CYP proteins in populations of stem and progenitor cells from different species as the CYP3A2 (a rat form similar to human CYP3A4) was shown to be expressed in bone marrow derived cells by RT-PCR (Avital et al. 2001)."@en . "Presence of Cytochrome P450 Enzymes in Human CD34(+) Haematopoietic Progenitor Cells" . "251-257" . "Anzenbacherov\u00E1, Eva" . . "23" . . . "6"^^ . "15110" . "7"^^ . "Kousalov\u00E1, E." . "Presence of Cytochrome P450 Enzymes in Human CD34(+) Haematopoietic Progenitor Cells" . "Presence of Cytochrome P450 Enzymes in Human CD34(+) Haematopoietic Progenitor Cells"@en . . "Baranov\u00E1, J." . . . "Human CD34(+) cells (haematopoietic stem and progenitor cells separated from peripheral blood) were shown to express CYP2E1 protein by Western blotting. For the first time, the specific CYP2E1 activity (chlorzoxazone 6-hydroxylation) was also detected. No CYP3A4 protein neither the CYP3A-specific nifedipine oxidase activity were detectable. The results obtained indicate differences in content of active CYP proteins in populations of stem and progenitor cells from different species as the CYP3A2 (a rat form similar to human CYP3A4) was shown to be expressed in bone marrow derived cells by RT-PCR (Avital et al. 2001)." . . . "Anzenbacher, Pavel" . . "581589" . . "Vondr\u00E1kov\u00E1, J." . . "2" . "Skoumalov\u00E1, I." . "SK - Slovensk\u00E1 republika" . "RIV/61989592:15110/04:00000899!RIV/2005/MZ0/151105/N" . "0231-5882" . . "Presence of Cytochrome P450 Enzymes in Human CD34(+) Haematopoietic Progenitor Cells"@en . "General Physiology and Biophysics" . "P\u0159\u00EDtomnost cytochrom\u016F P450 v lidsk\u00FDch CD34(+) hematopoetick\u00FDch progenitorov\u00FDch bu\u0148k\u00E1ch"@cs .