"Enterokoky se staly z\u00E1va\u017En\u00FDm probl\u00E9mem vzhledem ke sv\u00E9 etiologick\u00E9 roli v bakteri\u00E1ln\u00EDch infekc\u00EDch. Zvy\u0161uje se v\u00FDznamn\u011B pod\u00EDl enterokok\u016F s rezistenc\u00ED k vankomycinu a z tohoto d\u016Fvodu je \u0161\u00ED\u0159en\u00ED t\u011Bchto kmen\u016F velmi d\u016Fle\u017Eitou epidemiologickou problematikou. C\u00EDlem t\u00E9to studie bylo monitorovat enterokoky s rezistenc\u00ED k vankomycinu v \u010CR a prov\u00E9st jejich molekul\u00E1rn\u011B biologickou anal\u00FDzu."@cs . "Molekul\u00E1rn\u011B-biologick\u00E1 anal\u00FDza vankomycin-rezistentn\u00EDch enterokok\u016F izolovan\u00FDch z komunitn\u00EDho prost\u0159ed\u00ED \u010Cesk\u00E9 republiky"@cs . "RIV/61989592:15110/04:00000892!RIV/2005/MZ0/151105/N" . . "Molecular-biological analysis of vancomycin-resistant enterococci isolated from a community in the Czech Republic" . "Koukalov\u00E1, Dagmar" . . "CZ - \u010Cesk\u00E1 republika" . "V\u00E1gnerov\u00E1, Iva" . "574407" . "Hejnar, Petr" . . . . "P(NI7305)" . "15110" . . "2" . "Biomedical Papers" . . "Molekul\u00E1rn\u011B-biologick\u00E1 anal\u00FDza vankomycin-rezistentn\u00EDch enterokok\u016F izolovan\u00FDch z komunitn\u00EDho prost\u0159ed\u00ED \u010Cesk\u00E9 republiky"@cs . "3"^^ . "[12370BE94954]" . "148" . "Molecular-biological analysis of vancomycin-resistant enterococci isolated from a community in the Czech Republic" . . "RIV/61989592:15110/04:00000892" . "Molecular-biological analysis of vancomycin-resistant enterococci isolated from a community in the Czech Republic"@en . . . "\u010Cekanov\u00E1, Lubom\u00EDra" . "Vancomycin-resistant;enterococci;community;Czech Republic"@en . . "Kol\u00E1\u0159, Milan" . "7"^^ . . "Kesselov\u00E1, Michaela" . "1213-8118" . "Sauer, Pavel" . . . . "Objective: To determine the occurence of vancomycin-resistant enterococci (VRE) in a community of the Czech Republic and a molecular-biological analysis of the VRE isolated. Methods: Enterococci were isolated from the rectal swabs of healthy people in the Olomouc region (population 300,000), Czech Republic in the period of Januar - December 2003. The molecular-biological analysis of VRE was performed by analysis of isolated DNA, which was cleaved by restriction enzyme Smal and separated by pulse field gel electrophoresis (PFGE). Results: A total number of 5,283 swabs were evaluated and 558 Enterococcus sp. strains were isolated during the follow-up period. 9 strains (1.6%) were identified as VRE. Two strains were E. faecium phenotype VanA, one strain was E. faecalis phenotype VanB, two strains were E. gallinarum phenotype VanC and four strains were E. casseliflavus phenotype VanC. PFGE was used to obtain 9 different restriction profiles of VRE strains. The analysis showed closer a sim" . . "Molecular-biological analysis of vancomycin-resistant enterococci isolated from a community in the Czech Republic"@en . "Objective: To determine the occurence of vancomycin-resistant enterococci (VRE) in a community of the Czech Republic and a molecular-biological analysis of the VRE isolated. Methods: Enterococci were isolated from the rectal swabs of healthy people in the Olomouc region (population 300,000), Czech Republic in the period of Januar - December 2003. The molecular-biological analysis of VRE was performed by analysis of isolated DNA, which was cleaved by restriction enzyme Smal and separated by pulse field gel electrophoresis (PFGE). Results: A total number of 5,283 swabs were evaluated and 558 Enterococcus sp. strains were isolated during the follow-up period. 9 strains (1.6%) were identified as VRE. Two strains were E. faecium phenotype VanA, one strain was E. faecalis phenotype VanB, two strains were E. gallinarum phenotype VanC and four strains were E. casseliflavus phenotype VanC. PFGE was used to obtain 9 different restriction profiles of VRE strains. The analysis showed closer a sim"@en . "167-169" . . "4"^^ . . .