. . "Sanguinarine (SA), a member of the benzo[c]phenanthridine isoquinoline alkaloids, has been shown to possess antimicrobial, anti-inflammatory, and antioxidant properties. We examined the effects of SA on oxidative burst in DMSO-differentiated HL-60 cells, an excellent model for studying oxidative burst. SA inhibited both N-formyl-Met-Leu-Phe (fMLP) and phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst with half-maximal concentration for inhibition (IC(50)) of 1.5 and 1.8 microM, respectively. Despite suggestions of SA antioxidant activity this inhibition cannot be ascribed to radical scavenging property of SA because the IC(50) for superoxide dismutase-like activity in a non-cellular system was 60 microM. TROLOX, a water-soluble vitamin E analog, had IC(50) of 3 microM in the same system. Moreover, cyclic voltammetry measurements show that SA is not an easily oxidizable species, with a peak anodic potential at 700 mV, as compared to TROLOX with peak anodic potential at 200 mV. On the other" . "Sanguinarine (SA), a member of the benzo[c]phenanthridine isoquinoline alkaloids, has been shown to possess antimicrobial, anti-inflammatory, and antioxidant properties. We examined the effects of SA on oxidative burst in DMSO-differentiated HL-60 cells, an excellent model for studying oxidative burst. SA inhibited both N-formyl-Met-Leu-Phe (fMLP) and phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst with half-maximal concentration for inhibition (IC(50)) of 1.5 and 1.8 microM, respectively. Despite suggestions of SA antioxidant activity this inhibition cannot be ascribed to radical scavenging property of SA because the IC(50) for superoxide dismutase-like activity in a non-cellular system was 60 microM. TROLOX, a water-soluble vitamin E analog, had IC(50) of 3 microM in the same system. Moreover, cyclic voltammetry measurements show that SA is not an easily oxidizable species, with a peak anodic potential at 700 mV, as compared to TROLOX with peak anodic potential at 200 mV. On the other"@en . "Hrb\u00E1\u010D, Jan" . . "Vrba, Ji\u0159\u00ED" . . "RIV/61989592:15110/04:00000854" . "Sanguinarin (SA) je benzo[c]fenanthridinov\u00FD alkaloid isochinolinov\u00E9ho typu s antimikrobi\u00E1ln\u00EDmi, protiz\u00E1n\u011Btliv\u00FDmi a antioxida\u010Dn\u00EDmi vlastnostmi. Studovali jsme vliv SA na oxidativn\u00ED vzplanut\u00ED bun\u011Bk HL-60 diferencovan\u00FDch dimethylsulfoxidem na neutrolily. SA inhiboval vzplanut\u00ED aktivovan\u00E9 peptidem N-formyl-Met-Leu-Phe (fMLP) a 12-O-myristoylforbol-13-acet\u00E1tem (PMA), p\u0159i\u010Dem\u017E p\u0159\u00EDslu\u0161n\u00E9 hodnoty IC50 byly 1,5 a 1,8 #M. Tato inhibice nebyla zp\u016Fsobena vychyt\u00E1v\u00E1n\u00EDm radik\u00E1l\u016F, co\u017E bylo potvrzeno tak\u00E9 vylou\u010Den\u00EDm oxidovatelnost SA metodou cyklick\u00E9 voltametrie. P\u0159\u00EDm\u00FD vliv SA na NADPH-oxidasu v postgranul\u00E1rn\u00ED frakci z diferencovan\u00FDch bun\u011Bk HL-60 vyk\u00E1zal inhibici s hodnotou IC50 8,3 #M. Zv\u00FD\u0161en\u00ED hodnoty vzhledem k hodnot\u00E1m z\u00EDskan\u00FDm v bun\u011B\u010Dn\u00E9m syst\u00E9mu lze vysv\u011Btlit vlivem SDS. Z v\u00FDsledk\u016F usuzujeme, \u017Ee inhibice oxidativn\u00EDho vzplanut\u00ED nen\u00ED zp\u016Fsobena redoxn\u00ED aktivitou SA, ale pravd\u011Bpodobn\u011B je vyvol\u00E1na p\u0159\u00EDm\u00FDm vlivem SA na NADPH-oxidasu a \u010D\u00E1ste\u010Dn\u011B potla\u010Den\u00EDm tvorby enzymov\u00E9ho komplexu NADPH-oxidasy."@cs . . "Ulrichov\u00E1, Jitka" . "Sanguinarine is a potent inhibitor of oxidative burst in DMSO-differentiated HL-60 cells by a non-redox mechanism" . . "IE - Irsko" . "Sanguinarine is a potent inhibitor of oxidative burst in DMSO-differentiated HL-60 cells by a non-redox mechanism"@en . . "13"^^ . . . "Sanguinarine is a potent inhibitor of oxidative burst in DMSO-differentiated HL-60 cells by a non-redox mechanism" . "RIV/61989592:15110/04:00000854!RIV/2005/MSM/151105/N" . "Modriansk\u00FD, Martin" . "Sanguinarin je \u00FA\u010Dinn\u00FDm inhibitorem oxidativn\u00EDho vzplanut\u00ED nereduk\u010Dn\u00EDm mechanismem v DMSO-diferencovan\u00FDch bu\u0148k\u00E1ch HL-60"@cs . "Sanguinarin je \u00FA\u010Dinn\u00FDm inhibitorem oxidativn\u00EDho vzplanut\u00ED nereduk\u010Dn\u00EDm mechanismem v DMSO-diferencovan\u00FDch bu\u0148k\u00E1ch HL-60"@cs . "35-47" . . . "Sanguinarine;oxidative burst;NADPH oxidase;cyclic voltammetry;redox activity"@en . "[8B434089C04B]" . . . . . . "585450" . . "0009-2797" . "4"^^ . "15110" . "3"^^ . "Sanguinarine is a potent inhibitor of oxidative burst in DMSO-differentiated HL-60 cells by a non-redox mechanism"@en . "1" . . "147" . . "Chemico-Biological Interactions" . "Z(MSM 151100003)" .