. "Dvo\u0159\u00E1k, Zden\u011Bk" . "Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes" . "Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes"@en . "5"^^ . . "9"^^ . "15110" . . "Toxicology in Vitro" . "The aims of the present study were (1) to determine the cytotoxicity of colchiceine (EIN) in comparison with that of colchicine (COL); (2) to evaluate the effect of EIN on cytochrome P450 (CYP) expression and activity. Primary human hepatocytes were the model of choice for cytotoxicity and CYP expression experiments. LDH leakage and albumin secretion served as cytotoxicity parameters. EIN was less toxic than COL based on both parameters within the concentration range 1-100 microM. 10 microM concentration of EIN did not induce the expression of CYP1A2, CYP2A6, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 isoforms, which were evaluated at the levels of mRNAs, proteins and specific activities in culture. EIN in concentrations up to 200 microM had no effect on marker activities of CYP1A2, 2C9, 2E1 and 3A4 in human liver microsomes. It was concluded that EIN in concentrations up to 10 microM is not cytotoxic in primary human hepatocytes as revealed by albumin secretion and LDH leakage. Possible drug-drug interaction"@en . "Pichard-Garcia, Lydiane" . "Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes" . . "The aims of the present study were (1) to determine the cytotoxicity of colchiceine (EIN) in comparison with that of colchicine (COL); (2) to evaluate the effect of EIN on cytochrome P450 (CYP) expression and activity. Primary human hepatocytes were the model of choice for cytotoxicity and CYP expression experiments. LDH leakage and albumin secretion served as cytotoxicity parameters. EIN was less toxic than COL based on both parameters within the concentration range 1-100 microM. 10 microM concentration of EIN did not induce the expression of CYP1A2, CYP2A6, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 isoforms, which were evaluated at the levels of mRNAs, proteins and specific activities in culture. EIN in concentrations up to 200 microM had no effect on marker activities of CYP1A2, 2C9, 2E1 and 3A4 in human liver microsomes. It was concluded that EIN in concentrations up to 10 microM is not cytotoxic in primary human hepatocytes as revealed by albumin secretion and LDH leakage. Possible drug-drug interaction" . . "Srovn\u00E1n\u00ED \u00FA\u010Dinku kolchicinu a kolchiceinu na cytotoxicitu a expresi CYP gen\u016F v prim\u00E1rn\u00EDch kultur\u00E1ch lidsk\u00FDch hepatocyt\u016F"@cs . . . "3" . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "16" . . "Modriansk\u00FD, Martin" . "Ulrichov\u00E1, Jitka" . "RIV/61989592:15110/02:00006985!RIV09-MSM-15110___" . . . "0887-2333" . "641241" . . . "RIV/61989592:15110/02:00006985" . . . "Srovn\u00E1n\u00ED \u00FA\u010Dinku kolchicinu a kolchiceinu na cytotoxicitu a expresi CYP gen\u016F v prim\u00E1rn\u00EDch kultur\u00E1ch lidsk\u00FDch hepatocyt\u016F"@cs . . . . "3"^^ . "[BFFAD868A921]" . . "COL); (2) zhodnotit \u00FA\u010Dinek EIN na expresi a aktivitu cytochromu P450 (CYP). Prim\u00E1rn\u00ED lidsk\u00E9 hepatocyty byly zvoleny jako model pro experimenty zam\u011B\u0159en\u00E9 na cytotoxicitu a expresi CYP. Uvoln\u011Bn\u00ED LDH a sekrece albuminu slou\u017Eily jako parametry cytotoxicity. EIN byl m\u00E9n\u011B toxick\u00FD ne\u017E COL vzhledem k ob\u011Bma parametr\u016Fm a koncentrac\u00EDm v rozsahu 1-100 mikromol\u00E1rn\u00ED. 10 mikromol\u00E1rn\u00ED koncentrace EIN nezvy\u0161ovala expresi isoforem CYP1A2, CYP2A6, CYP2C9, CYP2C19, CYP2E1 a CYP3A4, kter\u00E9 byly hodnoceny na \u00FArovni mRNA, proteinu a specifick\u00E9 aktivity. EIN v koncentrac\u00EDch do 200 mikromol\u00E1rn\u00ED nem\u011Bl \u00FA\u010Dinek na aktivity CYP1A2, 2C9, 2E1 a 3A4 v mikrosom\u00E1ln\u00ED frakci lidsk\u00FDch hepatocyt\u016F. Z\u00E1v\u011Brem lze \u0159\u00EDci, \u017Ee EIN v koncentrac\u00EDch do 10 mikromol\u00E1rn\u00ED je netoxick\u00FD v prim\u00E1rn\u00EDch lidsk\u00FDch hepatocytech, jak prok\u00E1zala sekrece albuminu a uvoln\u011Bn\u00ED LDH. Mo\u017En\u00E9 mezil\u00E9kov\u00E9 interakce EIN d\u00EDky \u00FA\u010Dink\u016Fm na cytochromy P450 1A2, 2C9, 2E1 a 3A4 jsou nepravd\u011Bpodobn\u00E9, proto\u017Ee inhibi\u010Dn\u00ED a induk\u010Dn\u00ED studie \u017E\u00E1dn\u00E9 \u00FA\u010Dinky neprok\u00E1zaly. Jeliko\u017E EIN vykazuje lep\u0161\u00ED"@cs . "Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes"@en . "P(GA303/99/P002), Z(MSM 151100003)" . "Maurel, Patrick" . "Colchiceine; colchicine; cytotoxicity; cytochrome P450; human hepatocyte"@en .