. . "Oxidative damage of DNA induced in plasmid by the reactions with chemical agents"@en . . . "Antioxidants; DNA damage; Plasmid; Reactive oxygen species; Strand breaks"@en . "Oxidative damage of DNA induced in plasmid by the reactions with chemical agents" . "The aim of the work was to study interactions of chemical agents with nucleic acids. A wide variety of chemicals can cause damage of macromolecules, particularly oxidative stress leads to DNA damage. In this study, we tested chemicals that can cause oxidative DNA damage at sugar-phosphate backbone level. For the study of the effects of chemical agents on DNA plasmid DNA was used. The method using plasmid DNA was based on the different mobility of various topological forms of plasmid DNA. The changes in conformation were detected by means of agarose gel electrophoresis. Significant increases in formation of DNA strand breaks in plasmid DNA were detected after incubation of plasmid DNA with KMnO4, K2S2O8, peroxiacetic acid and NaClO. The results also show the effect of metal ions on DNA damage. In the present work, the effects of the agents with promising antioxidant activity (N-acetyl-cysteine, mannitol, dimethylsulfoxide, etc.) on plasmid DNA strand breakage has been studied." . "Oxidative damage of DNA induced in plasmid by the reactions with chemical agents" . . "385709" . "RIV/61988987:17310/08:A0900RJ1" . . . "The aim of the work was to study interactions of chemical agents with nucleic acids. A wide variety of chemicals can cause damage of macromolecules, particularly oxidative stress leads to DNA damage. In this study, we tested chemicals that can cause oxidative DNA damage at sugar-phosphate backbone level. For the study of the effects of chemical agents on DNA plasmid DNA was used. The method using plasmid DNA was based on the different mobility of various topological forms of plasmid DNA. The changes in conformation were detected by means of agarose gel electrophoresis. Significant increases in formation of DNA strand breaks in plasmid DNA were detected after incubation of plasmid DNA with KMnO4, K2S2O8, peroxiacetic acid and NaClO. The results also show the effect of metal ions on DNA damage. In the present work, the effects of the agents with promising antioxidant activity (N-acetyl-cysteine, mannitol, dimethylsulfoxide, etc.) on plasmid DNA strand breakage has been studied."@en . . "Pavl\u00ED\u010Dkov\u00E1, Zuzana" . "Environmental changes and biological assessment IV" . . . "3"^^ . . "391"^^ . "[930C7AD61BD1]" . . . . "17310" . "RIV/61988987:17310/08:A0900RJ1!RIV09-MSM-17310___" . "3"^^ . "S" . "Oxida\u010Dn\u00ED po\u0161kozen\u00ED DNA indukovan\u00E9 v plasmidu DNA reakc\u00ED s chemick\u00FDmi l\u00E1tkami"@cs . "978-80-7368-451-8" . "Pe\u010Dinka, Petr" . "Ostravsk\u00E1 univerzita v Ostrav\u011B" . "Oxidative damage of DNA induced in plasmid by the reactions with chemical agents"@en . "Oxida\u010Dn\u00ED po\u0161kozen\u00ED DNA indukovan\u00E9 v plasmidu DNA reakc\u00ED s chemick\u00FDmi l\u00E1tkami"@cs . "5"^^ . . . "C\u00EDlem pr\u00E1ce bylo studium interakc\u00ED plasmidu DNA s chemick\u00FDmi l\u00E1tkami, testov\u00E1na byla skupina oxida\u010Dn\u00EDch l\u00E1tek, kter\u00E9 mohou indukovat po\u0161kozen\u00ED DNA na \u00FArovni cukr-fosf\u00E1tov\u00E9ho \u0159et\u011Bzce. Pro detekci p\u0159eru\u0161en\u00ED cukr-fosf\u00E1tov\u00E9ho \u0159et\u011Bzce jsme pou\u017Eili gelovou elektrofor\u00E9zu, d\u016Fsledkem \u0161t\u011Bpen\u00ED \u0159et\u011Bzce je vznik zlomu v molekule DNA, co\u017E se projev\u00ED zm\u011Bnou topologie DNA. Z testovan\u00FDch l\u00E1tek byla v\u00FDrazn\u00E1 zm\u011Bna topologie plasmidov\u00E9 DNA pozorov\u00E1na u KMnO4, K2S2O8, kyseliny peroxoctov\u00E9 a NaClO. Dal\u0161\u00EDm d\u00EDl\u010D\u00EDm c\u00EDlem bylo zam\u011B\u0159it se na problematiku po\u0161kozen\u00ED DNA voln\u00FDmi radik\u00E1ly, v souvislosti se schopnost\u00ED iont\u016F tranzitn\u00EDch kov\u016F v p\u0159\u00EDtomnosti peroxidu vod\u00EDku generovat hydroxylov\u00E9 radik\u00E1ly a zp\u016Fsobovat tak oxida\u010Dn\u00ED po\u0161kozen\u00ED DNA. V t\u00E9to studii jsme z\u00EDskali prvn\u00ED zku\u0161enosti p\u0159i vyu\u017Eit\u00ED t\u00E9to metody pro detekci antioxida\u010Dn\u00EDch \u00FA\u010Dink\u016F l\u00E1tek. Inhibi\u010Dn\u00ED \u00FA\u010Dinek oxida\u010Dn\u00EDho po\u0161kozen\u00ED DNA byl detekov\u00E1n u mannitolu a dimethylsulfoxidu, zat\u00EDmco N-acetyl-cystein indukoval zv\u00FD\u0161en\u00ED tvorby dvou\u0159et\u011Bzcov\u00FDch zlom\u016F."@cs . "Ostrava" . "\u010Cerve\u0148, Ji\u0159\u00ED" . . .