. "RIV/61988987:17310/04:A1000V4D!RIV10-MSM-17310___" . . . "0950-9232" . "Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding ( SSDB) of human wild- type ( wt) p53 protein. We found that supercoiled ( sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20- mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the target sequence were the best competitors; removal of the cruciforms resulted in a decrease of competitor strength. In contrast to the full- length wt p53, the deletion mutant p53CDelta30 and the p53 core domain ( 93 - 312 aa) showed no enhancement of p53 SSDB to scDNA, suggesting that, in addition to the p53 core domain, the C- terminal was involved in this binding."@en . "1"^^ . "RIV/61988987:17310/04:A1000V4D" . . "8"^^ . . . "Karlovsk\u00E1, Lenka" . . . . "Br\u00E1zdov\u00E1, Marie" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "12" . . "[142DB35D9AFD]" . "23" . "Pale\u010Dek, Emil" . . . . "P(GA301/00/D001), P(IAA4004110), P(IAB5004203), P(OC D21.002), V, Z(AV0Z5004920)" . . "17310" . . . "ONCOGENE" . "Enhancement of p53 sequence-specific binding by DNA supercoiling" . "Br\u00E1zda, V\u00E1clav" . . "Pe\u010Dinka, Petr" . "Jagelsk\u00E1, Eva" . . . . "Enhancement of p53 sequence-specific binding by DNA supercoiling"@en . . "562906" . "Enhancement of p53 sequence-specific binding by DNA supercoiling" . . "6"^^ . . "competition assay; p53; DNA sequence-specific binding; supercoiled DNA; cruciform extrusion"@en . "Enhancement of p53 sequence-specific binding by DNA supercoiling"@en . "Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding ( SSDB) of human wild- type ( wt) p53 protein. We found that supercoiled ( sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20- mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the target sequence were the best competitors; removal of the cruciforms resulted in a decrease of competitor strength. In contrast to the full- length wt p53, the deletion mutant p53CDelta30 and the p53 core domain ( 93 - 312 aa) showed no enhancement of p53 SSDB to scDNA, suggesting that, in addition to the p53 core domain, the C- terminal was involved in this binding." .