. "Baloun, Ji\u0159\u00ED" . "Protoplasma" . "AT - Rakousk\u00E1 republika" . . . "Nevrtalov\u00E1, Eva" . "9"^^ . . "0033-183X" . . "Hobza, Roman" . "Dole\u017Eel, Jaroslav" . "Copper; Gene duplication; Metallothionein"@en . . . . "13"^^ . . "251" . "Vyskot, Boris" . "2"^^ . "Milde, D." . . . . "000344336500013" . "Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes" . . "\u010Cegan, Radim" . . "Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress." . "Hudzieczek, Vojt\u011Bch" . "I, P(ED0007/01/01), P(ED2.1.00/03.0058), P(GA522/09/0083), P(GAP501/12/2220), P(GBP501/12/G090), P(GP13-34962P), S" . "16167" . . "Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress."@en . . . "Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes"@en . "10.1007/s00709-014-0644-x" . "Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes"@en . . "[4DC280C25C15]" . "6" . . . "RIV/61389030:_____/14:00440405!RIV15-GA0-61389030" . . "RIV/61389030:_____/14:00440405" . "\u0160af\u00E1\u0159, Jan" . "Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes" . . .