. . "Chromosome Analysis and Sorting Using Flow Cytometry" . . "Kubal\u00E1kov\u00E1, Marie" . "5"^^ . . . "329"^^ . . "P(GA521/07/1573), P(LC06004), Z(AV0Z50380511)" . . . "Chromosome Analysis and Sorting Using Flow Cytometry"@en . . . . "5"^^ . . . "RIV/61389030:_____/11:00375427!RIV12-AV0-61389030" . "Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure for preparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can be induced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications."@en . "Humana Press" . "Chromosome Analysis and Sorting Using Flow Cytometry" . . . "10.1007/978-1-61737-957-4_12" . "Plant Chromosome Engineering: Methods and Protocols" . . . "Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure for preparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can be induced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications." . . "Dole\u017Eel, Jaroslav" . . "New Jersey" . . "[B32634A98363]" . "978-1-61737-956-7" . "18"^^ . . "\u0160imkov\u00E1, Hana" . "Chromosome analysis; flow cytometry; root meristems"@en . "Such\u00E1nkov\u00E1, Pavla" . . "\u010C\u00EDhal\u00EDkov\u00E1, Jarmila" . "RIV/61389030:_____/11:00375427" . . "190340" . "Chromosome Analysis and Sorting Using Flow Cytometry"@en .