"First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach" . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . "10.1371/journal.pone.0026421" . "Forcato, C." . "First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach"@en . . . . "Kubal\u00E1kov\u00E1, Marie" . "First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach" . "3"^^ . . "\u0160imkov\u00E1, Hana" . . . "Vitulo, N." . . . . . "000296186900060" . "RIV/61389030:_____/11:00366036" . "Cattivelli, L." . "Wheat is one of the world\u2019s most important crops and is characterized by a large polyploid genome. One way to reduce genome complexity is to isolate single chromosomes using flow cytometry. Low coverage DNA sequencing can provide a snapshot of individual chromosomes, allowing a fast characterization of their main features and comparison with other genomes. We used massively parallel 454 pyrosequencing to obtain a 2x coverage of wheat chromosome 5A. The resulting sequence assembly was used to identify TEs, genes and miRNAs, as well as to infer a virtual gene order based on the synteny with other grass genomes. Repetitive elements account for more than 75% of the genome. Gene content was estimated considering non-redundant reads showing at least one match to ESTs or proteins. The results indicate that the coding fraction represents 1.08% and 1.3% of the short and long arm respectively, projecting the number of genes of the whole chromosome to approximately 5,000."@en . "wheat chromosome 5A; Chromosome arm sorting; DNA amplification"@en . "199863" . "RIV/61389030:_____/11:00366036!RIV12-AV0-61389030" . . "Perrotta, G." . "[30735D9D62C7]" . "Wheat is one of the world\u2019s most important crops and is characterized by a large polyploid genome. One way to reduce genome complexity is to isolate single chromosomes using flow cytometry. Low coverage DNA sequencing can provide a snapshot of individual chromosomes, allowing a fast characterization of their main features and comparison with other genomes. We used massively parallel 454 pyrosequencing to obtain a 2x coverage of wheat chromosome 5A. The resulting sequence assembly was used to identify TEs, genes and miRNAs, as well as to infer a virtual gene order based on the synteny with other grass genomes. Repetitive elements account for more than 75% of the genome. Gene content was estimated considering non-redundant reads showing at least one match to ESTs or proteins. The results indicate that the coding fraction represents 1.08% and 1.3% of the short and long arm respectively, projecting the number of genes of the whole chromosome to approximately 5,000." . "6" . "Dole\u017Eel, Jaroslav" . "1932-6203" . "First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach"@en . "14"^^ . . "P(ED0007/01/01), Z(AV0Z50380511)" . . "21"^^ . "Albiero, A." . "PLoS ONE" . . . "10" . .