"Vereecke, D." . "Strnad, Miroslav" . . . "246534" . . . "RIV/61389030:_____/10:00359308!RIV11-GA0-61389030" . "Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry"@en . . . "Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry" . "Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry" . "A sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete Rhodococcus fascians. The cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (C18 and ion-exchange). The purified samples were further separated and analyzed by HPLC-ESI-MS/MS. This allowed to achieve chromatographic resolution of six highly hydrophobic cytokinin species including 2-methylthio-isopentenyladenine, 2-methylthio-isopentenyladenosine, 2-methylthio-trans-zeatin and 2-methylthio-trans-zeatin riboside and their cis-isomers. Quantification was performed by a standard isotope dilution method using a multiple-reaction monitoring (MRM) mode." . "Cytokinins; Rhodococcus fascians; High-performance liquid chromatography"@en . "Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry"@en . "680" . . "Analytica Chimica Acta" . "000284385800013" . "[89978DBFC3CF]" . . "0003-2670" . . "P(GA301/08/1649), Z(AV0Z50380511), Z(MSM6198959216)" . . . "Hanu\u0161, Jan" . "Nov\u00E1k, Ond\u0159ej" . "5"^^ . "Pertry, I." . . "9"^^ . . "\u0160ebela, M." . . "Tarkowski, Petr" . . . "V\u00E1clav\u00EDkov\u00E1, Kate\u0159ina" . "Whenham, R." . "6"^^ . "A sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete Rhodococcus fascians. The cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (C18 and ion-exchange). The purified samples were further separated and analyzed by HPLC-ESI-MS/MS. This allowed to achieve chromatographic resolution of six highly hydrophobic cytokinin species including 2-methylthio-isopentenyladenine, 2-methylthio-isopentenyladenosine, 2-methylthio-trans-zeatin and 2-methylthio-trans-zeatin riboside and their cis-isomers. Quantification was performed by a standard isotope dilution method using a multiple-reaction monitoring (MRM) mode."@en . . . "1-2" . "NL - Nizozemsko" . . "RIV/61389030:_____/10:00359308" . . . .