"Changes in metabolism of spinach (Spinacia oleraceae L., cv. Matador) plants exposed to stress caused by two heavy metals, cadmium and zinc, were studied. The distribution of these elements in biomass (root, leaf blade, petiole), chlorophyll content and activity of glutamate kinase [E.C.2.7.2.11], the enzyme catalyzing the first step of proline biosynthesis, were investigated in pot experiments. Results of the experiment revealed the toxic effects of cadmium and zinc at both tested levels (2.5, 25 mg Cd and 50, 500 mg Zn . kg-1 soil) for spinach. Under these conditions, decrease of glutamate kinase activity in spinach plants grown on contaminated treatments compared to untreated control was found. Allosteric regulation of proline content was investigated due to the synthesis of glutathione and subsequently phytochelatines in plant cells. The higher doses of both metals negatively affected yield of spinach dry biomass." . . "5-6" . . "PL - Polsk\u00E1 republika" . . . "Bal\u00EDk, J." . "RIV/61389030:_____/09:00338331!RIV10-AV0-61389030" . "16" . . "Sz\u00E1kov\u00E1, J." . "toxic metals; regulation of proline"@en . "1231-7098" . "7"^^ . . "Pavl\u00EDkov\u00E1, D." . "6"^^ . "The effect of cadmium and zinc on metabolism of spinach"@en . . "[42A635E2D727]" . "Chemia i inzynieria ekologiczna" . "The effect of cadmium and zinc on metabolism of spinach"@en . . "Tlusto\u0161, P." . "Z(AV0Z50380511)" . . "1"^^ . "312021" . "The effect of cadmium and zinc on metabolism of spinach" . "The effect of cadmium and zinc on metabolism of spinach" . . . . . "RIV/61389030:_____/09:00338331" . "Staszkov\u00E1, L." . "Changes in metabolism of spinach (Spinacia oleraceae L., cv. Matador) plants exposed to stress caused by two heavy metals, cadmium and zinc, were studied. The distribution of these elements in biomass (root, leaf blade, petiole), chlorophyll content and activity of glutamate kinase [E.C.2.7.2.11], the enzyme catalyzing the first step of proline biosynthesis, were investigated in pot experiments. Results of the experiment revealed the toxic effects of cadmium and zinc at both tested levels (2.5, 25 mg Cd and 50, 500 mg Zn . kg-1 soil) for spinach. Under these conditions, decrease of glutamate kinase activity in spinach plants grown on contaminated treatments compared to untreated control was found. Allosteric regulation of proline content was investigated due to the synthesis of glutathione and subsequently phytochelatines in plant cells. The higher doses of both metals negatively affected yield of spinach dry biomass."@en . "Pavl\u00EDk, Milan" . .