. . "Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley" . "B M C Genomics" . . . . "H\u0159ibov\u00E1, Eva" . . "Bhat, P. R." . "[0AA6D4A8F927]" . "P(GD521/05/H013), P(LC06004), P(ME 884), Z(AV0Z50380511)" . . "Vyu\u017Eit\u00ED DNA amplifikovan\u00E9 z t\u0159\u00EDd\u011Bn\u00FDch chromoz\u00F3m\u016F pro mapov\u00E1n\u00ED SNA marker\u016F u je\u010Dmene"@cs . "294" . "Pr\u016Ftokov\u00E1 cytometrie umo\u017E\u0148uje t\u0159\u00EDd\u011Bn\u00ED chromoz\u00F3m\u016F, kter\u00E9 mohou b\u00FDt vyu\u017Eity p\u0159i anal\u00FDze genomu. Z\u00EDsk\u00E1n\u00ED mikrogramov\u00FDch mno\u017Estv\u00ED DNA vy\u017Eaduje vyt\u0159\u00EDd\u011Bn\u00ED mili\u00F3n\u016F chromoz\u00F3m\u016F, co\u017E je pracn\u00E9 a \u010Dasov\u011B n\u00E1ro\u010Dn\u00E9. Mnoho genomick\u00FDch aplikac\u00ED, jako je v\u00FDvoj genetick\u00FDch map nebo fyzick\u00E9 mapov\u00E1n\u00ED, v\u0161ak nevy\u017Eaduje dlouh\u00E9 fragmenty DNA. V takov\u00FDch p\u0159\u00EDpadech m\u016F\u017Ee b\u00FDt doba t\u0159\u00EDd\u011Bn\u00ED minimalizovan\u00E1 vyu\u017Eit\u00EDm celogenomov\u00E9 amplifikace. V t\u00E9to pr\u00E1ci je pops\u00E1n protokol pro amplifikaci DNA z deseti tis\u00EDc chromoz\u00F3m\u016F je\u010Dmene, kter\u00E9 mohou b\u00FDt vyt\u0159\u00EDd\u011Bny za m\u00E9n\u011B ne\u017E jednu hodinu. Srov\u00E1n\u00ED p\u0159\u00EDtomnosti SNP marker\u016F v amplifikovan\u00E9 a neamplikovan\u00E9 DNA potvrdilo vysokou reprezentativnost amplifikace. Tato metoda podstatn\u011B roz\u0161i\u0159uje mo\u017Enosti vyu\u017Eit\u00ED t\u0159\u00EDd\u011Bn\u00FDch chromoz\u00F3m\u016F v genomice rostlin."@cs . "9" . "Background: Flow cytometry facilitates sorting of single chromosomes and chromosome arms which can be used for targeted genome analysis. However, the recovery of microgram amounts of DNA needed for some assays requires sorting of millions of chromosomes which is laborious and time consuming. Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification. Results: Here we report a protocol optimized in barley including amplification of DNA from only ten thousand chromosomes, which can be isolated in less than one hour. Flow-sorted chromosomes were treated with proteinase K and amplified using Phi29 multiple displacement amplification (MDA). Overnight amplification in a 20-microlitre reaction produced 3.7 \u2013 5.7 micrograms DNA with a majority of products between 5 and 30 kb. To determine the purity of sorted fractions and potential amplifica..."@en . "Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley"@en . "9"^^ . . "Dole\u017Eel, Jaroslav" . "\u0160af\u00E1\u0159, Jan" . . "RIV/61389030:_____/08:00322569" . . . "Background: Flow cytometry facilitates sorting of single chromosomes and chromosome arms which can be used for targeted genome analysis. However, the recovery of microgram amounts of DNA needed for some assays requires sorting of millions of chromosomes which is laborious and time consuming. Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification. Results: Here we report a protocol optimized in barley including amplification of DNA from only ten thousand chromosomes, which can be isolated in less than one hour. Flow-sorted chromosomes were treated with proteinase K and amplified using Phi29 multiple displacement amplification (MDA). Overnight amplification in a 20-microlitre reaction produced 3.7 \u2013 5.7 micrograms DNA with a majority of products between 5 and 30 kb. To determine the purity of sorted fractions and potential amplifica..." . "\u0160imkov\u00E1, Hana" . . . . "RIV/61389030:_____/08:00322569!RIV09-AV0-61389030" . "Condamine, P." . . . . . "Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley"@en . "Vyu\u017Eit\u00ED DNA amplifikovan\u00E9 z t\u0159\u00EDd\u011Bn\u00FDch chromoz\u00F3m\u016F pro mapov\u00E1n\u00ED SNA marker\u016F u je\u010Dmene"@cs . "6"^^ . "Barto\u0161, Jan" . "361462" . . . . . "Svensson, J. T." . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Such\u00E1nkov\u00E1, Pavla" . "Close, T. J." . . "10"^^ . "000257741700001" . "Flow cytometry; DNA amplification; Hordeum vulgare L."@en . . "Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley" . "1471-2164" .