. "Hauserov\u00E1, Eva" . "[C435A0DE3B6A]" . "Dole\u017Eal, Karel" . . . . . . "Cytokinin profiling in plant tissues using ultra-performance liquid chromatography\u2013electrospray tandem mass spectrometry" . "0031-9422" . . "69" . "Strnad, Miroslav" . "Phytochemistry" . "Byla vyvinuta jednoduch\u00E1 imunoextrak\u010Dn\u00ED mikropurifika\u010Dn\u00ED metoda pro extrakci p\u0159irozen\u011B se vyskytuj\u00EDc\u00EDch cytokinin\u016F kombinovan\u00E1 s ultra-\u00FA\u010Dinnou kapalinovou chromatografi\u00ED spojenou s tandemovou hmotnostn\u00ED spektrometri\u00ED. Nov\u00E9 extrak\u010Dn\u00ED a purifika\u010Dn\u00ED postupy byly optimalizov\u00E1ny na vzorc\u00EDch topolov\u00E9ho list\u00ED a analytick\u00E1 p\u0159esnost validov\u00E1na na 10-ti denn\u00EDch semen\u00E1\u010Dc\u00EDch A. thaliana. Tento p\u0159\u00EDstup m\u016F\u017Ee b\u00FDt pou\u017Eit pro rychlou, citlivou kvalitativn\u00ED a/nebo kvantitativn\u00ED anal\u00FDzu v\u00EDc ne\u017E 50 p\u0159irozen\u00FDch cytokinin\u016F v mg mno\u017Estv\u00ED rostlinn\u00FDch tk\u00E1n\u00ED."@cs . "Cytokinin profiling in plant tissues using ultra-performance liquid chromatography\u2013electrospray tandem mass spectrometry"@en . "RIV/61389030:_____/08:00322259!RIV09-AV0-61389030" . . "P(KAN200380801), S, Z(AV0Z50380511), Z(MSM6198959216)" . "Ultra-performance liquid chromatography (UPLC); Tandem mass spectrometry (MS/MS); Microextraction"@en . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "11"^^ . "000258863100018" . "Cytokininov\u00E1 anal\u00FDza v rostlinn\u00FDch tk\u00E1n\u00EDch pomoc\u00ED ultra-\u00FA\u010Dinn\u00E9 kapalinov\u00E9 chromatografie-elektrosprej tandemov\u00E9 hmotnostn\u00ED spektrometrie"@cs . . "Nov\u00E1k, Ond\u0159ej" . "We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15\u20130.3% and 1.0\u20135.5%, respectively). In multiple reaction monitoring..."@en . . . . . "5"^^ . "Cytokininov\u00E1 anal\u00FDza v rostlinn\u00FDch tk\u00E1n\u00EDch pomoc\u00ED ultra-\u00FA\u010Dinn\u00E9 kapalinov\u00E9 chromatografie-elektrosprej tandemov\u00E9 hmotnostn\u00ED spektrometrie"@cs . "361889" . "5"^^ . . . "We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15\u20130.3% and 1.0\u20135.5%, respectively). In multiple reaction monitoring..." . "RIV/61389030:_____/08:00322259" . . . . "Cytokinin profiling in plant tissues using ultra-performance liquid chromatography\u2013electrospray tandem mass spectrometry" . . . . . . "11" . "Amakorov\u00E1, Petra" . "Cytokinin profiling in plant tissues using ultra-performance liquid chromatography\u2013electrospray tandem mass spectrometry"@en .