. "Byly sledov\u00E1ny zm\u011Bny v obsaz\u00EDch polyamin\u016F v suspenzn\u00EDch kultur\u00E1ch bun\u011Bk vojt\u011B\u0161ky (Medicago sativa L.) a tab\u00E1ku (Nicotiana tabacum L. bright yellow 2; BY-2) v pr\u016Fb\u011Bhu jejich r\u016Fstov\u00FDch cykl\u016F. Na celkov\u00E9 hladin\u011B polyamin\u016F se v bu\u0148k\u00E1ch vojt\u011B\u0161ky pod\u00EDlel vysok\u00FDm obsahem p\u0159edev\u0161\u00EDm voln\u00FD putrescin a celkov\u00FD obsah polyamin\u016F byl ve srovn\u00E1n\u00ED s tab\u00E1kem t\u00E9m\u011B\u0159 desetin\u00E1sobn\u00FD. Naopak, v bu\u0148k\u00E1ch tab\u00E1ku se putrescin vyskytoval p\u0159ev\u00E1\u017En\u011B v konjugovan\u00E9 form\u011B. V\u00FDrazn\u00E9 rozd\u00EDly byly pozorov\u00E1ny v mno\u017Estv\u00ED polyamin\u016F vylu\u010Dovan\u00FDch do r\u016Fstov\u00E9ho m\u00E9dia u obou bun\u011B\u010Dn\u00FDch kultur. Na po\u010D\u00E1tku lag-f\u00E1ze r\u016Fstov\u00E9ho cyklu suspenzn\u00ED kultury vojt\u011B\u0161ky p\u0159edstavovalo mno\u017Estv\u00ED vylou\u010Den\u00E9ho putrescinu 21%, spermidinu 38%, sperminu 12% a kadaverin 15% z celkov\u00E9ho obsahu polyamin\u016F, zat\u00EDmco v r\u016Fstov\u00E9m m\u00E9diu tab\u00E1ku pouze 12% putrescinu a 2,5% spermidinu z celkov\u00E9ho obsahu. V pr\u00E1ci je diskutov\u00E1na \u00FAloha exkrece polyamin\u016F na udr\u017Een\u00ED optim\u00E1ln\u00ED intracelul\u00E1rn\u00ED hladiny polyamin\u016F."@cs . . "Excretion of polyamines in alfalfa and tobacco suspension-cultured cells and its possible role in maintenance of intracellular polyamine contents" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . . "7" . . "[E3654F8D35C4]" . "Changes in polyamines (PAs) in cells and cultivation media of alfalfa (Medicago sativa L.) and tobacco bright yellow 2 (BY-2) (Nicotiana tabacum L.) cell suspension cultures were studied over their growth cycles. The total content of PAs (both free and conjugated forms) was nearly 10 times higher in alfalfa, with high level of free putrescine (Put) (in exponential growth phase it represented about 65\u201373% of the intracellular Put pool). In contrast, the high content of soluble Put conjugates was found in tobacco cells (in exponential phase about 70% of the intracellular Put). Marked differences occurred in the amount of PAs excreted into the cultivation medium: alfalfa cells excreted at the first day after inoculation 2117.0, 230.5, 29.0 and 88.0 nmol g1 of cell fresh weight (FW) of Put, spermidine (Spd), spermine (Spm) and cadaverine (Cad), respectively, while at the same time tobacco cells excreted only small amount of Put and Spd (12.7 and 2.4 nmol g1 FW, respectively). On day 1 the amounts of Pu..."@en . "10"^^ . "Exkrece polyamin\u016F v bun\u011B\u010Dn\u00FDch suspenzn\u00EDch kultur\u00E1ch vojt\u011B\u0161ky a tab\u00E1ku a jej\u00ED mo\u017En\u00E1 \u00FAloha p\u0159i udr\u017Een\u00ED intracelul\u00E1rn\u00EDho obsahu polyamin\u016F"@cs . . "RIV/61389030:_____/08:00320629!RIV09-AV0-61389030" . "Eder, Josef" . "Changes in polyamines (PAs) in cells and cultivation media of alfalfa (Medicago sativa L.) and tobacco bright yellow 2 (BY-2) (Nicotiana tabacum L.) cell suspension cultures were studied over their growth cycles. The total content of PAs (both free and conjugated forms) was nearly 10 times higher in alfalfa, with high level of free putrescine (Put) (in exponential growth phase it represented about 65\u201373% of the intracellular Put pool). In contrast, the high content of soluble Put conjugates was found in tobacco cells (in exponential phase about 70% of the intracellular Put). Marked differences occurred in the amount of PAs excreted into the cultivation medium: alfalfa cells excreted at the first day after inoculation 2117.0, 230.5, 29.0 and 88.0 nmol g1 of cell fresh weight (FW) of Put, spermidine (Spd), spermine (Spm) and cadaverine (Cad), respectively, while at the same time tobacco cells excreted only small amount of Put and Spd (12.7 and 2.4 nmol g1 FW, respectively). On day 1 the amounts of Pu..." . "Arginine decarboxylase; Diamine oxidase; Ornithine decarboxylase"@en . . . "367068" . "27" . "Excretion of polyamines in alfalfa and tobacco suspension-cultured cells and its possible role in maintenance of intracellular polyamine contents" . "Plant Cell Reports" . "P(IAA6038303), Z(AV0Z50380511)" . "Excretion of polyamines in alfalfa and tobacco suspension-cultured cells and its possible role in maintenance of intracellular polyamine contents"@en . . "4"^^ . . . . "4"^^ . . "RIV/61389030:_____/08:00320629" . "0721-7714" . . "Exkrece polyamin\u016F v bun\u011B\u010Dn\u00FDch suspenzn\u00EDch kultur\u00E1ch vojt\u011B\u0161ky a tab\u00E1ku a jej\u00ED mo\u017En\u00E1 \u00FAloha p\u0159i udr\u017Een\u00ED intracelul\u00E1rn\u00EDho obsahu polyamin\u016F"@cs . . "000256477200003" . "Cvikrov\u00E1, Milena" . "Za\u017E\u00EDmalov\u00E1, Eva" . . . "Gemperlov\u00E1, Lenka" . . "Excretion of polyamines in alfalfa and tobacco suspension-cultured cells and its possible role in maintenance of intracellular polyamine contents"@en . .