"Efficient regeneration via somatic embryogenesis (SE) would be a valuable system for the micropropagation and genetic transformation of sugar beet. This study evaluated the effects of basic culture media (MS and PGo), plant growth regulators, sugars and the starting plant material on somatic embryogenesis in nine sugar beet breeding lines. Somatic embryos were induced from seedlings of several genotypes via an intervening callus phase on PGo medium containing N6-benzylaminopurine (BAP). Calli were mainly induced from cotyledons. Maltose was more effective for the induction of somatic embryogenesis than was sucrose. There were significant differences between genotypes. HB 526 and SDM 3, which produced embryogenic calli at frequencies of 25\u201350%, performed better than SDM 2, 8, 9 and 11. The embryogenic calli and embryos produced by this method were multiplied by repeated subculture. Histological analysis of embryogenic callus cultures indicated that somatic embryos were derived from single- or a smal..." . . "Kubal\u00E1kov\u00E1, Marie" . "Sugar beet; somatic embryogenesis; culture medium"@en . "Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines" . . . "13"^^ . "NL - Nizozemsko" . "[6C0F3BE82767]" . . . "Elliott, M. C." . "0167-6857" . "000254751900010" . "2" . "Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines"@en . "Efektivn\u00ED somatick\u00E1 embryogeneze u \u0161lechtitelsk\u00FDch lini\u00ED cukrovky (Beta vulgaris L.)"@cs . . "Efektivn\u00ED regenerace pomoc\u00ED somatick\u00E9 embryogeneze (SE) by mohla b\u00FDt cenn\u00FDm syst\u00E9mem pro mikropropagaci a genetickou transformaci cukrov\u00E9 \u0159epy. Tato studie hodnot\u00ED efekt z\u00E1kladn\u00EDho kultiva\u010Dn\u00EDho media (MS a PGo), r\u016Fstov\u00FDch regul\u00E1tor\u016F, cukr\u016F a v\u00FDchoz\u00EDho rostlinn\u00E9ho materi\u00E1lu na embryogenezi u dev\u00EDti lini\u00ED cukrov\u00E9 \u0159epy. Somatick\u00E1 embrya byla indukov\u00E1na z kalus\u016F na PGo mediu s N6-benzylaminopurinem (BAP). Kalusy byly indukov\u00E1ny p\u0159ev\u00E1\u017En\u011B z d\u011Bloh. Malt\u00F3za byla pro indukci SE vhodn\u011Bj\u0161\u00ED ne\u017E sachar\u00F3za. Byly pozorov\u00E1ny signifikantn\u00ED rozd\u00EDly mezi genotypy. HB 526 a SDM 3, produkovaly embryogenn\u00ED kalusy ve frekvenci 25\u201350%, to je v\u00EDce ne\u017E SDM 2, 8, 9 a 11. Histologickou anal\u00FDzou embryogenn\u00EDho kalusu bylo zji\u0161t\u011Bno, \u017Ee somatick\u00E1 embrya vyr\u016Fstala z jednotliv\u00FDch bun\u011Bk nebo z mal\u00E9ho po\u010Dtu bun\u011Bk. Kyselina 2,4-dichlorophenoxyoctov\u00E1 (2,4-D) nebyla pro indukci somatick\u00E9 embryogeneze ze semen\u00E1\u010Dk\u016F efektivn\u00ED, ale indukovala p\u0159\u00EDmou somatickou embryogenezi z nezral\u00FDch zygotick\u00FDch embry\u00ED (IEs). Somatick\u00E1 embrya byla iniciov\u00E1..."@cs . "365506" . "RIV/61389030:_____/08:00320012!RIV09-AV0-61389030" . "P(LC06004), Z(AV0Z50380511)" . "Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines" . . "Zhang, C. L." . "Plant Cell, Tissue and Organ Culture" . "1"^^ . "Efektivn\u00ED somatick\u00E1 embryogeneze u \u0161lechtitelsk\u00FDch lini\u00ED cukrovky (Beta vulgaris L.)"@cs . . . "93" . . "Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines"@en . "Slater, A." . "RIV/61389030:_____/08:00320012" . . . "Zhang, J." . . . "Scott, N. W." . "7"^^ . "Efficient regeneration via somatic embryogenesis (SE) would be a valuable system for the micropropagation and genetic transformation of sugar beet. This study evaluated the effects of basic culture media (MS and PGo), plant growth regulators, sugars and the starting plant material on somatic embryogenesis in nine sugar beet breeding lines. Somatic embryos were induced from seedlings of several genotypes via an intervening callus phase on PGo medium containing N6-benzylaminopurine (BAP). Calli were mainly induced from cotyledons. Maltose was more effective for the induction of somatic embryogenesis than was sucrose. There were significant differences between genotypes. HB 526 and SDM 3, which produced embryogenic calli at frequencies of 25\u201350%, performed better than SDM 2, 8, 9 and 11. The embryogenic calli and embryos produced by this method were multiplied by repeated subculture. Histological analysis of embryogenic callus cultures indicated that somatic embryos were derived from single- or a smal..."@en . . . "Chen, D. F." . .