. "H\u00E1la, Michal" . "5" . . "Komplex Exocyst se \u00FA\u010Dastn\u00ED na r\u016Fstu rostlinn\u00E9 bu\u0148ky v Arabidopsis a tab\u00E1ku"@cs . . "355664" . "Synek, Luk\u00E1\u0161" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Plant Cell" . . "000257320600013" . "An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco"@en . "11"^^ . "Lamkemeyer, T." . "Drdov\u00E1, Edita" . . "16"^^ . "RIV/61389030:_____/08:00318543!RIV09-AV0-61389030" . "An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco" . . "5"^^ . "P(IAA6038410), P(LC06034), P(ME 841), Z(AV0Z50380511), Z(MSM0021620858)" . . "Pe\u010Denkov\u00E1, Tamara" . "\u017D\u00E1rsk\u00FD, Viktor" . . . "Cole, R. A." . . "1040-4651" . . . "Komplex Exocyst se \u00FA\u010Dastn\u00ED na r\u016Fstu rostlinn\u00E9 bu\u0148ky v Arabidopsis a tab\u00E1ku"@cs . . "An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco"@en . "Exocyst je oktamerick\u00FD poutac\u00ED komplex. Skl\u00E1d\u00E1 se z osmi podjednotek - SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 a EXO84. Prozat\u00EDm byl charakterizov\u00E1n na molekul\u00E1rn\u00ED \u00FArovni u kvasinek a \u017Eivo\u010Dich\u016F. V t\u00E9to pr\u00E1ci p\u0159in\u00E1\u0161\u00EDme genetick\u00E1, molekul\u00E1rn\u011B biologick\u00E1 a biochemick\u00E1 data ukazuj\u00EDc\u00ED, \u017Ee tento komplex funguje podobn\u00FDm zp\u016Fsobem tak\u00E9 v rostlinn\u00E9 bu\u0148ce, kde se pod\u00EDl\u00ED na kl\u00ED\u010Dov\u00FDch d\u011Bj\u00EDch pol\u00E1rn\u00EDho r\u016Fstu (kl\u00ED\u010Den\u00ED pylov\u00E9 l\u00E1\u010Dky, etiolovan\u00FD hypokotyl). Poda\u0159ilo se n\u00E1m tak\u00E9 uk\u00E1zat vz\u00E1jemn\u00E9 interakce jednotliv\u00FDch podjednotek, kter\u00E9 p\u0159in\u00E1\u0161ej\u00ED informaci o celkov\u00E9 struktu\u0159e komplexu."@cs . "20" . "[A4C81B2C3F58]" . . . . . "Hochholdinger, F." . . "EXOCYST; PROTEIN COMPLEX; CELL POLARITY; MORPHOGENESIS; GTPASES"@en . . . . "The exocyst, an octameric tethering complex and effector of Rho and Rab GTPases, facilitates polarized secretion in yeast and animals. Recent evidence implicates three plant homologs of exocyst subunits (SEC3, SEC8, and EXO70A1) in plant cell morphogenesis. Here, we provide genetic, cell biological, and biochemical evidence that these and other predicted subunits function together in vivo in Arabidopsis thaliana. Double mutants in exocyst subunits (sec5 exo70A1 and sec8 exo70A1) show a synergistic defect in etiolated hypocotyl elongation. Mutants in exocyst subunits SEC5, SEC6, SEC8, and SEC15a show defective pollen germination and pollen tube growth phenotypes. Using antibodies directed against SEC6, SEC8, and EXO70A1, we demonstrate colocalization of these proteins at the apex of growing tobacco pollen tubes. The SEC3, SEC5, SEC6, SEC8, SEC10, SEC15a, and EXO70 subunits copurify in a high molecular mass fraction of 900 kD after chromatographic fractionation of an Arabidopsis cell suspension extra..."@en . "RIV/61389030:_____/08:00318543" . . . "An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco" . . . "The exocyst, an octameric tethering complex and effector of Rho and Rab GTPases, facilitates polarized secretion in yeast and animals. Recent evidence implicates three plant homologs of exocyst subunits (SEC3, SEC8, and EXO70A1) in plant cell morphogenesis. Here, we provide genetic, cell biological, and biochemical evidence that these and other predicted subunits function together in vivo in Arabidopsis thaliana. Double mutants in exocyst subunits (sec5 exo70A1 and sec8 exo70A1) show a synergistic defect in etiolated hypocotyl elongation. Mutants in exocyst subunits SEC5, SEC6, SEC8, and SEC15a show defective pollen germination and pollen tube growth phenotypes. Using antibodies directed against SEC6, SEC8, and EXO70A1, we demonstrate colocalization of these proteins at the apex of growing tobacco pollen tubes. The SEC3, SEC5, SEC6, SEC8, SEC10, SEC15a, and EXO70 subunits copurify in a high molecular mass fraction of 900 kD after chromatographic fractionation of an Arabidopsis cell suspension extra..." . "Nordheim, A." . . "Madlung, J." . "Fowler, J. E." .