"\u0160olcov\u00E1, Blanka" . "P(GA206/02/0967), P(IAA600380507), Z(AV0Z50380511)" . "Phytochemistry" . "Zv\u00FD\u0161en\u00E9 vyu\u017E\u00EDv\u00E1n\u00ED modern\u00EDch metod, zejm\u00E9na hmotnostn\u00ED spektrometrie, pro stanoven\u00ED cytokinin\u016F zv\u00FD\u0161ilo po\u017Eadavky na extrakci a \u010Di\u0161t\u011Bn\u00ED t\u011Bchto rostlinn\u00FDch hormon\u016F. Porovn\u00E1n\u00ED t\u0159\u00ED r\u016Fzn\u00FDch extrak\u010Dn\u00EDch sm\u011Bs\u00ED (80% [v/v] metanolu, Bieleskiho MCF-7 sm\u011Bsi a modifikovan\u00E9 Bieleskiho sm\u011Bsi) uk\u00E1zalo, \u017Ee po extrakci modifikovanou sm\u011Bs\u00ED byly dosa\u017Eeny nejvy\u0161\u0161\u00ED odezvy deuterovan\u00FDch standard\u016F p\u0159i stanoven\u00ED cytokinin\u016F pomoc\u00ED hmotnosn\u00ED spektromerie (HPLC/MS/MS). \u010Ci\u0161t\u011Bn\u00ED cytokinin\u016F na sorbentu Oasis MCX (katex + reversn\u00ED f\u00E1ze) poskytovalo vy\u0161\u0161\u00ED v\u00FDt\u011B\u017Eky ribosidu zeatinu a jeho monofosf\u00E1tu a podobn\u00E9 v\u00FDt\u011B\u017Eky cytokininov\u00FDch b\u00E1z\u00ED, ribosid\u016F a glukosid\u016F ve srovn\u00E1n\u00ED s jejich \u010Di\u0161t\u011Bn\u00EDm chromatografi\u00ED na reversn\u00ED f\u00E1zi a DEAE Sephadexu. \u010Ci\u0161t\u011Bn\u00EDm na sorbentu Oasis MCX byl sn\u00ED\u017Een obsah kontaminuj\u00EDc\u00EDch l\u00E1tek absorbuj\u00EDc\u00EDch UV z\u00E1\u0159en\u00ED o 90%. Tato metoda se uk\u00E1zala jako vhodn\u00E1 pro \u010Dist\u011Bn\u00ED cytokinin\u016F p\u0159ed jejich stanoven\u00EDm pomoc\u00ED HPLC/MS/MS jak z hlediska jej\u00ED jednoduchosti, tak \u010Distoty prepar\u00E1t\u016F."@cs . "Kam\u00EDnek, Miroslav" . "Gaudinov\u00E1, Alena" . "11" . . "RIV/61389030:_____/06:00082323" . "Efficiency of different methods of extraction and purification of cytokinins"@en . . . "1151;1159" . "Efficiency of different methods of extraction and purification of cytokinins" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Malbeck, Ji\u0159\u00ED" . "[0C116EEB6457]" . . "8"^^ . . "\u00DA\u010Dinnost r\u016Fzn\u00FDch metod extrakce a \u010Di\u0161t\u011Bn\u00ED cytokinin\u016F"@cs . . . "7"^^ . "Efficiency of different methods of extraction and purification of cytokinins" . "The increasing use of advanced methods, such as mass spectrometry, for the determination of cytokinins has raised special requirements for the extraction and purification of this class of plant hormones. Extraction of Arabidopsis thaliana plants with three different solvents, [80% (v/v) MeOH, Bieleski\u2019s MCF-7, and modified Bieleski\u2019s] provided similar yields of most analyzed cytokinins determined by high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS). However, the extraction with a modified Bieleski\u2019s solvent (MeOH\u2013HCO2H\u2013H2O [15:1:4, v/v/v]) gave the highest responses of deuterated cytokinins (used as test compounds) in plant extracts as compared to the responses of pure deuterated standards (relative internal standard response, RISR). Purification of cytokinins using Oasis MCX sorbent with reversed-phase and cation-exchange characteristics, in comparison to the DEAE Sephadex RP-C18 method, provided higher levels of zeatin riboside monophosphate and similar levels of cytoki..."@en . . "Efficiency of different methods of extraction and purification of cytokinins"@en . "0031-9422" . "Hoyerov\u00E1, Kl\u00E1ra" . "67" . . "Tr\u00E1vn\u00ED\u010Dkov\u00E1, Alena" . . . "9"^^ . . . "The increasing use of advanced methods, such as mass spectrometry, for the determination of cytokinins has raised special requirements for the extraction and purification of this class of plant hormones. Extraction of Arabidopsis thaliana plants with three different solvents, [80% (v/v) MeOH, Bieleski\u2019s MCF-7, and modified Bieleski\u2019s] provided similar yields of most analyzed cytokinins determined by high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS). However, the extraction with a modified Bieleski\u2019s solvent (MeOH\u2013HCO2H\u2013H2O [15:1:4, v/v/v]) gave the highest responses of deuterated cytokinins (used as test compounds) in plant extracts as compared to the responses of pure deuterated standards (relative internal standard response, RISR). Purification of cytokinins using Oasis MCX sorbent with reversed-phase and cation-exchange characteristics, in comparison to the DEAE Sephadex RP-C18 method, provided higher levels of zeatin riboside monophosphate and similar levels of cytoki..." . "473411" . "Koc\u00E1bek, Tom\u00E1\u0161" . . . . . "RIV/61389030:_____/06:00082323!RIV07-AV0-61389030" . . . "Dobrev, Petre" . . "Arabidopsis thaliana; Cytokinin extraction; Cytokinin purification"@en . . . "\u00DA\u010Dinnost r\u016Fzn\u00FDch metod extrakce a \u010Di\u0161t\u011Bn\u00ED cytokinin\u016F"@cs . .