. . . "Busquets, A." . . "RIV/61389030:_____/06:00082318!RIV07-AV0-61389030" . "Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis" . "491255" . "Closa, M." . . . "0167-4412" . "Arabidopsis thaliana; cytokinin; farnesyl diphosphate synthase; isoprenoid"@en . "Arr\u00F3, M." . . . "61" . . . "[7AC30171F8BC]" . . "Plant Molecular Biology" . "Ferrer, A." . "Manzano, D." . "Kam\u00EDnek, Miroslav" . "NL - Nizozemsko" . "Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis"@en . "2"^^ . "Schaller, H." . "Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis"@en . . . "Hoyerov\u00E1, Kl\u00E1ra" . . . . "To investigate the role of mitochondrial farnesyl diphosphate synthase (FPS) in plant isoprenoid biosynthesis we characterized transgenic Arabidopsis thaliana plants overexpressing FPS1L isoform. This overexpressed protein was properly targeted to mitochondria yielding a mature and active form of the enzyme of 40 kDa. Leaves from transgenic plants grown under continuous light exhibited symptoms of chlorosis and cell death correlating to H2O2 accumulation, and leaves detached from the same plants displayed accelerated senescence. Overexpression of FPS in mitochondria also led to altered leaf cytokinin profile, with a reduction in the contents of physiologically active trans-zeatin- and isopentenyladenine-type cytokinins and their corresponding riboside monophosphates as well as enhanced levels of cis-zeatin 7-glucoside and storage cytokinin O-glucosides. Overexpression of 3-hydroxy-3-methylglutaryl coenzyme A reductase did not prevent chlorosis in plants overexpressing FPS1L, but did rescue accelera..." . "Pro pozn\u00E1n\u00ED \u00FAlohy mitochondri\u00E1ln\u00ED farnesyl difosf\u00E1t synt\u00E1zy (FPS) v biosynt\u00E9ze isoprenoid\u016F jsme pou\u017Eili rostlin Arabidospsis se zv\u00FD\u0161enou expres\u00ED jej\u00ED mitochondri\u00E1ln\u00ED isoformy (FPS1L). Bylo potvrzeno, \u017Ee p\u0159\u00EDslu\u0161n\u00E1 aktivn\u00ED 40 kDa b\u00EDlkovina je c\u00EDlen\u00E1 do mitochondri\u00ED. Transgenn\u00ED rostliny p\u011Bstovan\u00E9 za nep\u0159etr\u017Eit\u00E9ho osv\u011Btlen\u00ED vykazovaly symptomy chlor\u00F3zy a bun\u011B\u010Dn\u00E9 smrti, kter\u00E9 korelovaly s produkc\u00ED H2O2. V odd\u011Blen\u00FDch listech byla urychlena jejich senescence. Byl rovn\u011B\u017E zm\u011Bn\u011Bn profil cytokinin\u016F \u2013 byla sn\u00ED\u017Eena akumulace fyziologicky aktivn\u00EDch cytokinin\u016F typu trans-zeatinu a isopentenyladeninu. Sou\u010Dasn\u011B zv\u00FD\u0161ena exprese redukt\u00E1zy 3-hydroxy-3-metylglutaryl koenzymu A sice nezabr\u00E1nila chlor\u00F3ze, ale zpomalila senescenci a vr\u00E1tila hladiny cytokinin\u016F na \u00FArove\u0148 netransformovan\u00FDch rostlin. Domn\u00EDv\u00E1me se, \u017Ee zv\u00FD\u0161en\u00E1 exprese FPS1L vede k zv\u00FD\u0161en\u00E9 spot\u0159eb\u011B isopentenyl difosf\u00E1tu v mitochondri\u00EDch a touto cestou m\u011Bn\u00ED homeost\u00E1zi cytokinin\u016F pro jejich\u017E biosynt\u00E9zu je vyu\u017E\u00EDv\u00E1n spole\u010Dn\u00FD prekursor."@cs . "P(IAA600380507), Z(AV0Z50380511)" . "Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis" . "Zv\u00FD\u0161en\u00E1 exprese farnesyl difosf\u00E1t synt\u00E1zy vyvol\u00E1v\u00E1 na sv\u011Btle tvorbu l\u00E9z\u00ED a pozm\u011B\u0148uje homeost\u00E1zi cytokinin\u016F"@cs . "1-2" . . "8"^^ . "195;213" . "19"^^ . "To investigate the role of mitochondrial farnesyl diphosphate synthase (FPS) in plant isoprenoid biosynthesis we characterized transgenic Arabidopsis thaliana plants overexpressing FPS1L isoform. This overexpressed protein was properly targeted to mitochondria yielding a mature and active form of the enzyme of 40 kDa. Leaves from transgenic plants grown under continuous light exhibited symptoms of chlorosis and cell death correlating to H2O2 accumulation, and leaves detached from the same plants displayed accelerated senescence. Overexpression of FPS in mitochondria also led to altered leaf cytokinin profile, with a reduction in the contents of physiologically active trans-zeatin- and isopentenyladenine-type cytokinins and their corresponding riboside monophosphates as well as enhanced levels of cis-zeatin 7-glucoside and storage cytokinin O-glucosides. Overexpression of 3-hydroxy-3-methylglutaryl coenzyme A reductase did not prevent chlorosis in plants overexpressing FPS1L, but did rescue accelera..."@en . "RIV/61389030:_____/06:00082318" . . . "Zv\u00FD\u0161en\u00E1 exprese farnesyl difosf\u00E1t synt\u00E1zy vyvol\u00E1v\u00E1 na sv\u011Btle tvorbu l\u00E9z\u00ED a pozm\u011B\u0148uje homeost\u00E1zi cytokinin\u016F"@cs .