"Produkce polyklon\u00E1ln\u00EDch protil\u00E1tek proti rekombinantn\u00EDmu nestruktur\u00E1ln\u00EDmu proteinu 1 z \u201Etriple gene blocku\u201C mop-top viru bramboru"@cs . "Filigarov\u00E1, Marie" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . "Pe\u010Denkov\u00E1, Tamara" . "The gene encoding the triple gene protein 1 (TGBp1) of Potato mop-top protein (PMTV) was cloned into expression vector pQE32 tagging the protein with 6xHis on the N-terminus. When the gene was enshortened on its 3\u00B4-end by two different restriction digestions, efficient and high yield bacterial expression was achieved in both cases, as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PHAGE) and Western blot. One of these two constructs was used for raising rabbit polyclonal antibodies. The obtained sera and antibodies were tested for the detection of PMTV in laboratory host Nicotiana bethamiana and natural host Solanum tuberosum by enzyme-linked immunosorbent assay (ELISA) as well as by Western blota. The obtainad antisera are more suitable for Western blot analysis of infected plants than for ELSA." . "-" . . . . "The gene encoding the triple gene protein 1 (TGBp1) of Potato mop-top protein (PMTV) was cloned into expression vector pQE32 tagging the protein with 6xHis on the N-terminus. When the gene was enshortened on its 3\u00B4-end by two different restriction digestions, efficient and high yield bacterial expression was achieved in both cases, as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PHAGE) and Western blot. One of these two constructs was used for raising rabbit polyclonal antibodies. The obtained sera and antibodies were tested for the detection of PMTV in laboratory host Nicotiana bethamiana and natural host Solanum tuberosum by enzyme-linked immunosorbent assay (ELISA) as well as by Western blota. The obtainad antisera are more suitable for Western blot analysis of infected plants than for ELSA."@en . . "0931-1785" . "Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l" . "422;427" . . "[428C2F6924C0]" . "P(GA522/04/1329), Z(AV0Z50380511)" . "Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l"@en . "6"^^ . . "154" . . "RIV/61389030:_____/06:00041855" . "\u010Ce\u0159ovsk\u00E1, Noemi" . "Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l"@en . . "3"^^ . "Potato mop-top virus; recombinant protein; triple gene block; polyclonal antibodies"@en . . "Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l" . "495189" . . "3"^^ . "Produkce polyklon\u00E1ln\u00EDch protil\u00E1tek proti rekombinantn\u00EDmu nestruktur\u00E1ln\u00EDmu proteinu 1 z \u201Etriple gene blocku\u201C mop-top viru bramboru"@cs . . "Produkce polyklon\u00E1ln\u00EDch protil\u00E1tek proti rekombinantn\u00EDmu nestruktur\u00E1ln\u00EDmu proteinu 1 z \u201Etriple gene blocku\u201C mop-top viru bramboru. Gen koduj\u00EDc\u00ED protein 1 z \u201Etriple gene block\u201C (TGBp1) mop-top viru bramboru byl klonov\u00E1n v expresivn\u00EDm vektoru pQE32. Pomoc\u00ED postupn\u00E9ho zkracov\u00E1n\u00ED genu na jeho 3 konci dv\u011Bma r\u016Fzn\u00FDmi restrik\u010Dn\u00EDmi digescemi bylo u obou konstrukt\u016F dosa\u017Eeno vysok\u00E9 bakteri\u00E1ln\u00ED exprese. Jeden z t\u011Bchto konstrukt\u016F byl pou\u017Eit pro produkci kr\u00E1li\u010D\u00EDch polyklon\u00E1ln\u00EDch protil\u00E1tek. Z\u00EDskan\u00E1 antis\u00E9ra a protil\u00E1tky byly testov\u00E1ny na p\u0159\u00EDtomnost PMTV v laboratorn\u00EDm hostiteli Nicotina bentamiana a na p\u0159irozen\u00E9m hostiteli Solanum tuberosum . Z\u00EDskan\u00E1 antis\u00E9ra jsou vhodn\u011Bj\u0161\u00ED pro identifikaci infek\u010Dn\u00EDch rostlin metodou Western blotu ne\u017E pro metodu ELISA."@cs . . . "RIV/61389030:_____/06:00041855!RIV07-AV0-61389030" . . . "Journal of Phytopathology - Phytopathologische Zeitschrift" . . . .