. "P(GA522/04/1329), Z(AV0Z50380511)" . "Pe\u010Denkov\u00E1, Tamara" . . "To obtain strong bacterial expression of proteins that seem to be hard to express in bacteria or are highly toxic for bacteria, it is possible to vreate a palette of similar constructs, differing only by several nucleotides, gradually deleted from the full-lenght clone by ewonuclease III. When a construct is equipped with the 6xHis tag, a simple colony-blot procedure can be performed and a colony giving strong and efficient expression can easily sbe selected for high range protein expression. We utilized this procedure to produce one of potato mop-top virus (PMTV) movement proteins, namely triple gene block protein 1 (TGPp1) which was very hard toexpress in bacteria in its original length. The TGPp1 gene was digested with exonuclease III and nuclease S1 from its 5\u00B4 terminus. Leaving 6xHis tag intact. The clone that showed the strongest signal with anti-His antibodies in colony-blot procedure was found to have 44 amino acids (of total 463) deleted. The SDS-PAGE and Western blot of h..."@en . "[539542483D0D]" . "\u00DA\u010Dinn\u00E1 bakteri\u00E1ln\u00ED exprese rekombinantn\u00EDho nestruktur\u00E1ln\u00EDho proteinu 1 z \u201Etriple gene blocku\u201C mop-top viru bramboru"@cs . "Byla vypracov\u00E1na metoda pro expresi t\u011B\u017Eko exprimovateln\u00FDch \u010Di toxick\u00FDch protein\u016F v bakteri\u00EDch. Pomoc\u00ED postupn\u00E9ho zkracov\u00E1n\u00ED exonukleasou III a nukleasou S1 z 5\u00B4konce se vytvo\u0159ila \u0161k\u00E1la podobn\u00FDch konstrukt\u016F li\u0161\u00EDc\u00EDch se pouze o n\u011Bkolik nukleotid\u016F. Konstrukty byly vybaveny 6 x His kotvou, kter\u00E1 umo\u017Enila jejich detekci v colony- blotu. Pro expresi ve velk\u00E9m m\u011B\u0159\u00EDtku se pot\u00E9 vybraly kolonie poskytuj\u00EDc\u00ED dostate\u010Dn\u011B silnou expresi. Tato metoda byla vyu\u017Eita pro p\u0159\u00EDpravu jednoho z nestrukturn\u00EDch \u201Emovement\u201C protein\u016F mop-top viru bramboru (PMTV) ( prvn\u00EDho proteinu z \u201Etriple gene block\u201C (TGBp1)).Klon, kter\u00FD vykazoval nejsiln\u011Bj\u0161\u00ED expresi byl zkr\u00E1cen o 44 aminokyselin z celkov\u00FDch 463."@cs . . "Efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein 1 modified by progressive deletion of its N-terminus" . . "Efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein 1 modified by progressive deletion of its N-terminus" . "519657" . "To obtain strong bacterial expression of proteins that seem to be hard to express in bacteria or are highly toxic for bacteria, it is possible to vreate a palette of similar constructs, differing only by several nucleotides, gradually deleted from the full-lenght clone by ewonuclease III. When a construct is equipped with the 6xHis tag, a simple colony-blot procedure can be performed and a colony giving strong and efficient expression can easily sbe selected for high range protein expression. We utilized this procedure to produce one of potato mop-top virus (PMTV) movement proteins, namely triple gene block protein 1 (TGPp1) which was very hard toexpress in bacteria in its original length. The TGPp1 gene was digested with exonuclease III and nuclease S1 from its 5\u00B4 terminus. Leaving 6xHis tag intact. The clone that showed the strongest signal with anti-His antibodies in colony-blot procedure was found to have 44 amino acids (of total 463) deleted. The SDS-PAGE and Western blot of h..." . "Protein expression; Potato mop-top virus; Triple gene block"@en . "41" . . . "\u00DA\u010Dinn\u00E1 bakteri\u00E1ln\u00ED exprese rekombinantn\u00EDho nestruktur\u00E1ln\u00EDho proteinu 1 z \u201Etriple gene blocku\u201C mop-top viru bramboru"@cs . "RIV/61389030:_____/05:00028432!RIV06-GA0-61389030" . "\u010Ce\u0159ovsk\u00E1, Noemi" . . "Filigarov\u00E1, Marie" . . . "RIV/61389030:_____/05:00028432" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "128;135" . "8"^^ . . "Protein Expression and Purification" . "1046-5928" . . . "3"^^ . . "Efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein 1 modified by progressive deletion of its N-terminus"@en . . "Efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein 1 modified by progressive deletion of its N-terminus"@en . . "-" . . . "3"^^ . .