"Chromosome sorting in tetraploid wheat and its potential for genome analysis"@en . . . "823;829" . "Kubal\u00E1kov\u00E1, Marie" . "0016-6731" . . "7"^^ . "-" . "T\u0159\u00EDd\u011Bn\u00ED chromoz\u00F3m\u016F u tetraploidn\u00ED p\u0161enice a mo\u017Enosti pro anal\u00FDzu jej\u00EDho genomu"@cs . . "\u010C\u00EDhal\u00EDkov\u00E1, Jarmila" . . "Kov\u00E1\u0159ov\u00E1, Pavl\u00EDna" . . "Tato studie ukazuje mo\u017Enosti flow cytometrie pro t\u0159\u00EDd\u011Bn\u00ED chromoz\u00F3m\u016F p\u0161enice tvrd\u00E9 (Triti-cum turgidum Desf. var. durum,). Histogram intenzity fluorescence z DAPI obarven\u00FDch chromoz\u00F3m\u016F m\u00E1 t\u0159i p\u00EDky. Jeden tvo\u0159\u00ED chromoz\u00F3m 3B, mal\u00FD p\u00EDk zahrnuje chromoz\u00F3my 1A a 6A a v\u0161echny ostatn\u00ED chromoz\u00F3my jsou p\u0159edstavov\u00E1ny velk\u00FDm p\u00EDkem. Flow karyotyp dvoji-t\u00FDch telozomik\u016F ukazuje mo\u017Enost t\u0159\u00EDdit i v\u0161echna ramena chromoz\u00F3m\u016F a z nich vytvo\u0159it knihovny velk\u00FDch fragment\u016F. \u010Cl\u00E1nek ukazuje mo\u017Enost vyu\u017Eit\u00ED flow cytometrie pro genomiku p\u0161enice a sekvenov\u00E1n\u00ED jednotliv\u00FDch chromoz\u00F3mov\u00FDch ramen."@cs . "Dole\u017Eel, Jaroslav" . . . "Chromosome sorting in tetraploid wheat and its potential for genome analysis" . . . "Flow cytometry; chromosome sorting; wheat"@en . . . . "Barto\u0161, Jan" . "Chromosome sorting in tetraploid wheat and its potential for genome analysis"@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Lucretti, S." . . "Watanabe, N." . . "Kianian, S. F." . "515320" . "[435766CD987B]" . "T\u0159\u00EDd\u011Bn\u00ED chromoz\u00F3m\u016F u tetraploidn\u00ED p\u0161enice a mo\u017Enosti pro anal\u00FDzu jej\u00EDho genomu"@cs . . "9"^^ . . "Such\u00E1nkov\u00E1, Pavla" . . . "Genetics" . "RIV/61389030:_____/05:00026518!RIV06-GA0-61389030" . . "170" . . "RIV/61389030:_____/05:00026518" . "This study evaluates the potential of flow cytometry for chromosome sorting in durum wheat (Triticum turgidum Desf. var. durum,). Histograms of fluorescence intensity obtained after the analysis of DAPI-stained chromosomes consisted of three peaks. Of these, one represented chromosome 3B, a small peak corresponded to chromosomes 1A and 6A, and a large peak represented the remaining eleven chromosomes. Flow karyotyping in double ditelosomic lines of durum wheat revealed that the lines facilitated sorting of any arm of the wheat A- and B-genome chromosomes. We have demonstrated that large insert libraries can be created from DNA purified using flow cytometry. This study considerably expands the potential of flow cytogenetics for use in wheat genomics and opens the possibility of sequencing the genome of this important crop one chromosome arm at a time." . "P(GA521/04/0607), P(GA522/03/0354), Z(AV0Z50380511)" . "6"^^ . . "This study evaluates the potential of flow cytometry for chromosome sorting in durum wheat (Triticum turgidum Desf. var. durum,). Histograms of fluorescence intensity obtained after the analysis of DAPI-stained chromosomes consisted of three peaks. Of these, one represented chromosome 3B, a small peak corresponded to chromosomes 1A and 6A, and a large peak represented the remaining eleven chromosomes. Flow karyotyping in double ditelosomic lines of durum wheat revealed that the lines facilitated sorting of any arm of the wheat A- and B-genome chromosomes. We have demonstrated that large insert libraries can be created from DNA purified using flow cytometry. This study considerably expands the potential of flow cytogenetics for use in wheat genomics and opens the possibility of sequencing the genome of this important crop one chromosome arm at a time."@en . . "Chromosome sorting in tetraploid wheat and its potential for genome analysis" .