"RIV/61389030:_____/04:00109161!RIV/2005/AV0/A56005/N" . . "595782" . "5"^^ . "Z-DNA, a new in situ marker for transcription" . "Z-DNA forms transiently behind the active RNA polymerases, because of the mechanical torsional stress produced during transcription. In this paper, we explore the possibility that the distribution of Z-DNA stretches signals the sites related to nuclear transcription. To localize transcription, the in situ assay for active RNA polymerases, that allows the elongation of the already initiated transcripts but no initiation of new ones (run-on experiments), was carried out in isolated nuclei of Allium cepa L. root meristems. Both nucleolar and non-nucleolar sites appeared labelled. Nucleoli were most active in transcription than the multiple non-nucleolar foci altogether. In situ immunodetection of Z-DNA provided images that were comparable to those obtained after the run-on assay, with one exception: while Z-DNA and transcription sites were scattered throughout the whole nucleus, Z-DNA also accumulated in the nuclear periphery, where no transcription foci were detected in the run-on ass..." . "Allium cepa; Z-DNA immunodetection; in situ run-on transcription"@en . . . "7"^^ . . "Z-DNA, a new in situ marker for transcription."@cs . "RIV/61389030:_____/04:00109161" . . "Jouve, N." . "[0BFCF049B081]" . "48" . "IT - Italsk\u00E1 republika" . "Z-DNA, a new in situ marker for transcription"@cs . "P(IBS5038351), Z(AV0Z5038910)" . "1" . . "de la Espina, S. M. D." . . "\u010Cern\u00E1, Adriana" . "Cuadrado, A." . "Z-DNA forms transiently behind the active RNA polymerases, because of the mechanical torsional stress produced during transcription. In this paper, we explore the possibility that the distribution of Z-DNA stretches signals the sites related to nuclear transcription. To localize transcription, the in situ assay for active RNA polymerases, that allows the elongation of the already initiated transcripts but no initiation of new ones (run-on experiments), was carried out in isolated nuclei of Allium cepa L. root meristems. Both nucleolar and non-nucleolar sites appeared labelled. Nucleoli were most active in transcription than the multiple non-nucleolar foci altogether. In situ immunodetection of Z-DNA provided images that were comparable to those obtained after the run-on assay, with one exception: while Z-DNA and transcription sites were scattered throughout the whole nucleus, Z-DNA also accumulated in the nuclear periphery, where no transcription foci were detected in the run-on ass..."@en . "Z-DNA, a new in situ marker for transcription"@en . . . . "1"^^ . "Z-DNA, a new in situ marker for transcription"@en . "Z-DNA, a new in situ marker for transcription"@cs . . "1121-760X" . "49;55" . "EUROPEAN JOURNAL OF HISTOCHEMISTRY" . . . . "Z-DNA, a new in situ marker for transcription" . "De la Torre, C." . . . .