. "PLANT CELL AND ENVIRONMENT" . "P(IAA5038207), Z(AV0Z5038910), Z(MSM 113100003)" . . "586404" . . "RIV/61389030:_____/04:00109157" . "Cytoskelet\u00E1ln\u00ED b\u00EDlkoviny vytv\u00E1\u0159ej\u00ED dynamick\u00E9 polymery, kter\u00E9 se uplat\u0148uj\u00ED v mnoha procesech, v bun\u011B\u010Dn\u00E9m d\u011Blen\u00ED, sign\u00E1ln\u00ED transdukci a v determinaci tvaru a polarity bu\u0148ky. Distribuce a dynamika mikrotubul\u016F (MTs) a aktinov\u00FDch mikrofilament (AFs) jsou mimo jin\u00E9 determinov\u00E1ny lokalizac\u00ED jejich nuklea\u010Dn\u00EDch m\u00EDst. Zat\u00EDmco v\u00EDme, \u017Ee m\u00EDsto pro nukleaci rostlinn\u00FDch mitrotubul\u016F je pod plasmatickou membr\u00E1nou a b\u011Bhem interf\u00E1ze na jadern\u00E9m obalu, tak informace o nuklea\u010Dn\u00EDch m\u00EDstech AFs jsou zcela nedostate\u010Dn\u00E1. Tato pr\u00E1ce vyu\u017E\u00EDv\u00E1 n\u00EDzkou teplotu (0\u00B0C) k depolymeraci AFs a MTs u interf\u00E1zn\u00EDch BY-2 bun\u011Bk tab\u00E1ku (Nicotiana tabacum L.). Rozsah depolymerace cytoskelet\u00E1ln\u00EDch filament b\u011Bhem chladov\u00E9ho p\u016Fsoben\u00ED a subcelul\u00E1rn\u00ED distribuce nuklea\u010Dn\u00EDch m\u00EDst b\u011Bhem n\u00E1sledn\u00E9ho zotaven\u00ED v 25\u00B0C bylo hodnoceno fluorescen\u010Dn\u00ED mikroskopi\u00ED. V\u00FDsledky ukazuj\u00ED, \u017Ee AFs rychle repolymerovaly z m\u00EDst lokalizovan\u00FDch v kortik\u00E1ln\u00ED oblasti a na jadern\u00E9m obalu, tedy podobn\u011B jako MTs. Na rozd\u00EDl od MTs, p\u0159ed kompletn\u00ED rekostituc\u00ED A..."@cs . "5" . "M\u00EDsta iniciace a reorganizace aktinov\u00FDch filament v bu\u0148k\u00E1ch tab\u00E1ku po p\u016Fsoben\u00ED chladov\u00E9ho stresu"@cs . "Schwarzerov\u00E1, K." . . "2"^^ . "\u010Capkov\u00E1, V\u011Bra" . . . "Sites of actin filament initiation and reorganization in cold-treated tobacco cells"@en . . "Nicotiana tabacum; actin; actin filaments"@en . "7"^^ . . . "Janotov\u00E1, I." . . "Pokorn\u00E1, J." . "Petr\u00E1\u0161ek, Jan" . "Opatrn\u00FD, Z." . . . . . "13"^^ . "RIV/61389030:_____/04:00109157!RIV/2005/AV0/A56005/N" . . "Cytoskeletal proteins assemble into dynamic polymers that play many roles in nuclear and cell division, signal transduction, and determination of cell shape and polarity. The distribution and dynamics of microtubules (MTs) and actin filaments (AFs) are determined, among other factors, by the location of their nucleation sites. Whereas the sites of microtubule nucleation in plants are known to be located under the plasma membrane and on the nuclear envelope during interphase, there is a striking lack of information about nucleation sites of AFs. In the studies reported herein, low temperature (0degreesC) was used to de-polymerize AFs and MTs in tobacco BY-2 (Nicotiana tabacum L.) cells at interphase. The extent of de-polymerization of cytoskeletal filaments in interphase cells during cold treatment and the subcellular distribution of nucleation sites during subsequent recovery at 25degreesC were monitored by means of fluorescence microscopy. The results show that AFs re-polymerized r..."@en . "[BD971C324825]" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Zelenkov\u00E1, S." . "Sites of actin filament initiation and reorganization in cold-treated tobacco cells" . "27" . "Sites of actin filament initiation and reorganization in cold-treated tobacco cells"@en . "Cytoskeletal proteins assemble into dynamic polymers that play many roles in nuclear and cell division, signal transduction, and determination of cell shape and polarity. The distribution and dynamics of microtubules (MTs) and actin filaments (AFs) are determined, among other factors, by the location of their nucleation sites. Whereas the sites of microtubule nucleation in plants are known to be located under the plasma membrane and on the nuclear envelope during interphase, there is a striking lack of information about nucleation sites of AFs. In the studies reported herein, low temperature (0degreesC) was used to de-polymerize AFs and MTs in tobacco BY-2 (Nicotiana tabacum L.) cells at interphase. The extent of de-polymerization of cytoskeletal filaments in interphase cells during cold treatment and the subcellular distribution of nucleation sites during subsequent recovery at 25degreesC were monitored by means of fluorescence microscopy. The results show that AFs re-polymerized r..." . "0140-7791" . . "M\u00EDsta iniciace a reorganizace aktinov\u00FDch filament v bu\u0148k\u00E1ch tab\u00E1ku po p\u016Fsoben\u00ED chladov\u00E9ho stresu"@cs . . "641;653" . "Sites of actin filament initiation and reorganization in cold-treated tobacco cells" . .