"Puchta, H." . "Homolog husen\u00ED\u010Dku Rad17 se z\u00FA\u010Dast\u0148uje regulace reparace po\u0161kozen\u00ED DNA a homologn\u00ED rekombinace"@cs . . . . . "Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce AtRAD17 transcriptionally. AtRAD17 mutants show increased sensitivity to the DNA-damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9-1-1 complex (Rad9), which is also sensitive to the same chemicals. AtRAD9 and AtRAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double-strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants a..."@en . "1"^^ . "Hartung, F." . "RIV/61389030:_____/04:00109147!RIV/2005/AV0/A56005/N" . . "38" . "Plant Journal" . . . . . "6" . . . . . "RIV/61389030:_____/04:00109147" . "The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination"@en . . "Homolog husen\u00ED\u010Dku Rad17 se z\u00FA\u010Dast\u0148uje regulace reparace po\u0161kozen\u00ED DNA a homologn\u00ED rekombinace"@cs . . "[A5441092E5AC]" . "Heitzeberg, F." . "Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce AtRAD17 transcriptionally. AtRAD17 mutants show increased sensitivity to the DNA-damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9-1-1 complex (Rad9), which is also sensitive to the same chemicals. AtRAD9 and AtRAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double-strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants a..." . "15"^^ . "583503" . "Chen, I. P." . . "Orel, N." . "Arabidopsis; RAD17; RAD9"@en . "0960-7412" . "P(GA521/01/1418), P(ME 534), P(ME 535), Z(AV0Z5038910)" . "The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination" . "The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination"@en . "Rad17 vystupuje v kvasink\u00E1ch a lidsk\u00FDch bu\u0148k\u00E1ch jako kontroln\u00ED gen DNA. Jeho homolog stejn\u011B jako homology dal\u0161\u00EDch gen\u016F t\u00E9to dr\u00E1hy (9_1_1 komplex) se vyskytuje u husen\u00ED\u010Dku a sd\u00EDl\u00ED s nimi konzervovan\u00E9 sekvence a dom\u00E9ny. L\u00E1tky po\u0161kozuj\u00EDc\u00ED DNA indukuj\u00ED transkrip\u010Dn\u011B AtRAD17. Mutanty pak vykazuj\u00ED zv\u00FD\u0161enou citlivost k l\u00E1tk\u00E1m jako bleomycin nebo mitomycin C (MMC). Mutovan\u00FD fenotyp je kompenzov\u00E1n komplementac\u00ED, co\u017E nazna\u010Duje, \u017Ee ztr\u00E1ta funkce Rad17 naru\u0161uje reparaci DNA v rostlinn\u00FDch bu\u0148k\u00E1ch. Na\u0161e v\u00FDsledky potvrzuje tak\u00E9 fenotyp mutanta komplexu 9-1-1 (Rad9), kter\u00FD je citliv\u00FD ke stejn\u00FDm chemik\u00E1li\u00EDm. Ukazuje se, \u017Ee AtRAD9 a AtRAD17 jsou epistatick\u00E9, proto\u017Ee dvojit\u00FD mutant nen\u00ED citliv\u011Bj\u0161\u00ED ne\u017E samostatn\u00E9. Mutanty vykazuj\u00ED prodlevu obecn\u00E9 reparace dvojvl\u00E1knov\u00FDch zlom\u016F (DSB) DNA. Frekvence intrachromozom\u00E1ln\u00EDch homologi\u00EDch rekombinac\u00ED (HR) je v\u0161ak zv\u00FD\u0161ena. Nicm\u00E9n\u011B mutanty jsou proficientn\u00ED pro dal\u0161\u00ED indukci HR genotoxick\u00FDm stresem. Na\u0161e v\u00FDsledky nazna\u010Duj\u00ED, \u017Ee mutovan\u00E1 dr\u00E1ha Rad17 je spojena s celk..."@cs . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination" . "6"^^ . "Angelis, Karel" . . "954;968" . . .