"559205" . . . . . . "Strnad, Miroslav" . . "Mo\u017Enost prodlou\u017Een\u00ED p\u016Fsoben\u00ED exogenn\u00EDch cytokinin\u016F inhibic\u00ED jejich inaktivace byla studov\u00E1na v \u0159edkvi\u010Dce (Raphanus sativus L. cv. Rampouch). Vzhledem k tomu, \u017Ee v \u0159edkvi\u010Dce je hlavn\u00EDm mechanismem deaktivace cytokinin\u016F jejich N-glukosylace, studium se soust\u0159edilo na inhibitory t\u00E9to metabolick\u00E9 dr\u00E1hy - papaverin, theofylin a olomoucin, d\u00E1le na 2 strukturn\u00ED analoga olomoucinu - bohemin a roscovitin. Zjistili jsme, \u017Ee olomoucin, bohemin a roscovitin, kter\u00E9 jsou zn\u00E1my jako \u00FA\u010Dinn\u00E9 inhibitory cyklin-dependentn\u00EDch kinas, rovn\u011B\u017E efektivn\u011B inhibuj\u00ED N-glukosylaci cytokinin\u016F. Aplikace inhibitoru k nadzemn\u00EDm \u010D\u00E1stem kl\u00ED\u010Dn\u00EDch rostlin (prost\u0159ednictv\u00EDm vaskul\u00E1rn\u00EDho syst\u00E9mu) vedla po 24 hod k p\u0159ibli\u017En\u011B 50 % sn\u00ED\u017Een\u00ED konverze modelov\u00FDch cytokinin\u016F, [3H]dihydrozeatinu a [3H]N6-benzyladeninu, na p\u0159\u00EDslu\u0161n\u00E9 7N-glukosidy. Sou\u010Dasn\u011B byla zv\u00FD\u0161ena hladina nemetabolizovan\u00E9 base. Se vzr\u016Fstaj\u00EDc\u00ED koncentrac\u00ED inhibitor\u016F se sni\u017Eovala aktivita cytokinin oxidasy/dehydrogenasy (EC 1.5.99.12). Hladina fyziologicky aktivn..."@cs . "Inhibitory N-glukosylace cytokinin\u016F potla\u010Duj\u00ED deaktivaci exogenn\u00EDch cytokinin\u016F v \u0159edkvi\u010Dce, ale jejich efekt na endogenn\u00ED cytokininy je eliminov\u00E1n dal\u0161\u00EDmi regula\u010Dn\u00EDmi mechanismy"@cs . . . "121" . "The prolongation of the effect of exogenous cytokinins by inhibition of their inactivation was studied in Raphanus sativus L. cv. Rampouch. As in radish the main way of cytokinin inactivation is their N-glucosylation, inhibitors of this pathway, papaverine, theophylline and olomoucine, as well as two olomoucine analogues, bohemine and roscovitine, were tested. The latter ones, which function as potent inhibitors of cyclin-dependent kinases, have also been found to effectively inhibit cytokinin N-glucosylation. Incubation (24 h) of de-rooted radish seedlings with inhibitors resulted in c. 50% decrease of the conversion of model cytokinins, [3H]dihydrozeatin and [3H]N6-benzyladenine, to the corresponding 7N-glucosides. Simultaneously the level of the non-metabolized cytokinin bases was elevated. The activity of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) was suppressed in a dose dependent manner. The concentration of physiologically active endogenous cytokinins was not increased si..." . . . "RIV/61389030:_____/04:00104355" . "7"^^ . "Cytokinin N-glucosylation inhibitors suppress deactivation of exogenous cytokinins in radish, but their effect on active endogenous cytokinins is counteracted by other regulatory mechanisms" . "215;222" . "Va\u0148kov\u00E1, Radom\u00EDra" . . "8"^^ . "Inhibitory N-glukosylace cytokinin\u016F potla\u010Duj\u00ED deaktivaci exogenn\u00EDch cytokinin\u016F v \u0159edkvi\u010Dce, ale jejich efekt na endogenn\u00ED cytokininy je eliminov\u00E1n dal\u0161\u00EDmi regula\u010Dn\u00EDmi mechanismy"@cs . . . . . "Cytokinin N-glucosylation inhibitors suppress deactivation of exogenous cytokinins in radish, but their effect on active endogenous cytokinins is counteracted by other regulatory mechanisms"@en . "ABA; abscisic acid; N6-benzyladenine"@en . "7"^^ . "RIV/61389030:_____/04:00104355!RIV/2005/AV0/A56005/N" . . "2" . . "Cytokinin N-glucosylation inhibitors suppress deactivation of exogenous cytokinins in radish, but their effect on active endogenous cytokinins is counteracted by other regulatory mechanisms" . "Physiologia Plantarum" . "0031-9317" . . "DK - D\u00E1nsk\u00E9 kr\u00E1lovstv\u00ED" . "Blagoeva, Elitsa" . "The prolongation of the effect of exogenous cytokinins by inhibition of their inactivation was studied in Raphanus sativus L. cv. Rampouch. As in radish the main way of cytokinin inactivation is their N-glucosylation, inhibitors of this pathway, papaverine, theophylline and olomoucine, as well as two olomoucine analogues, bohemine and roscovitine, were tested. The latter ones, which function as potent inhibitors of cyclin-dependent kinases, have also been found to effectively inhibit cytokinin N-glucosylation. Incubation (24 h) of de-rooted radish seedlings with inhibitors resulted in c. 50% decrease of the conversion of model cytokinins, [3H]dihydrozeatin and [3H]N6-benzyladenine, to the corresponding 7N-glucosides. Simultaneously the level of the non-metabolized cytokinin bases was elevated. The activity of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) was suppressed in a dose dependent manner. The concentration of physiologically active endogenous cytokinins was not increased si..."@en . . . . . "P(GA206/03/0313), P(GA301/02/0475), P(GA522/99/1130), P(LN00A081), P(ME 505), Z(AV0Z5038910)" . "Motyka, V\u00E1clav" . "Dobrev, Petre" . "Cytokinin N-glucosylation inhibitors suppress deactivation of exogenous cytokinins in radish, but their effect on active endogenous cytokinins is counteracted by other regulatory mechanisms"@en . . . . "Malbeck, Ji\u0159\u00ED" . . "Hanu\u0161, Jan" . "[201DEC14F37C]" . .