. . . "Havl\u00ED\u010Dek, Libor" . . . . "Two 2,6,9-trisubstituted purine derivatives, 9-isopropyl-2,6-bis[(4-methoxybenzyl)amino]-9H-purine (myoseverin, PMYO, 1) and 9-isopropyl-2,6-bis[(2-methoxybenzyl)amino]-9H-purine (OMYO, 2), and two 6,9-disubstituted derivatives, 9-isopropyl-6-[(4-methoxybenzyl)amino]-9H-purine (3) and 9-isopropyl-6-[(2-methoxybenzyl)amino]-9H-purine (4), were synthesized with the aim to examine their cell proliferation inhibiting activity, and possible additional effects in cultures of hybridoma cells producing monoclonal antibody. The substances were tested over a concentration range from 0.003 to 30 mmol l-1. The most active compound 1 caused a total loss of cell viability at 1 mmol l-1, while its isomer 2 showed the same effect at 10 mmol l-1 concentration. In the presence of compound 1, but not of compound 2, the character of the cell cycle phases profile changed dramatically, most cells being arrested in the G2/M phase. At intermediate concentrations of compound 2 a substantially higher viable cell concentration" . "0010-0765" . "6"^^ . . "257;266" . . . "[BDCF9DC16C47]" . "Collection of Czechoslovak Chemical Communications" . . "Two 2,6,9-trisubstituted purine derivatives, 9-isopropyl-2,6-bis[(4-methoxybenzyl)amino]-9H-purine (myoseverin, PMYO, 1) and 9-isopropyl-2,6-bis[(2-methoxybenzyl)amino]-9H-purine (OMYO, 2), and two 6,9-disubstituted derivatives, 9-isopropyl-6-[(4-methoxybenzyl)amino]-9H-purine (3) and 9-isopropyl-6-[(2-methoxybenzyl)amino]-9H-purine (4), were synthesized with the aim to examine their cell proliferation inhibiting activity, and possible additional effects in cultures of hybridoma cells producing monoclonal antibody. The substances were tested over a concentration range from 0.003 to 30 mmol l-1. The most active compound 1 caused a total loss of cell viability at 1 mmol l-1, while its isomer 2 showed the same effect at 10 mmol l-1 concentration. In the presence of compound 1, but not of compound 2, the character of the cell cycle phases profile changed dramatically, most cells being arrested in the G2/M phase. At intermediate concentrations of compound 2 a substantially higher viable cell concentration"@en . "Eckschlager, T." . "RIV/61389030:_____/02:56023045" . "Siglerov\u00E1, V\u011Bra" . "P(GA301/02/0475), P(GA303/02/0875), P(OC 844.10), Z(AV0Z5038910)" . . . . "Purines; CDK Inhibitors; Cytokinins"@en . "67" . "644354" . "2" . "Fran\u011Bk, Franti\u0161ek" . . . "Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells." . . . . "CZ - \u010Cesk\u00E1 republika" . "0"^^ . "0"^^ . "Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells." . "10"^^ . "Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells."@en . . . . "Weigl, E." . "RIV/61389030:_____/02:56023045!RIV/2003/GA0/A56003/N" . "4"^^ . . "Strnad, Miroslav" . "Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells."@en .