"Filippov, Sergey" . "10.1021/jz400857z" . "Drechsler, M." . "[53F3F8CA6C85]" . "1948-7185" . . . "DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics" . "RIV/61389013:_____/13:00392998" . "70160" . . . . "Angelova, A." . "Couvreur, P." . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "000320214600034" . "I, P(GAP208/10/1600)" . "Narayanan, T." . "11" . "lipoplex; SAXS; cryo-TEM"@en . "3"^^ . "Structural changes occurring on a millisecond time scale during uptake of DNA by cationic lipid nanocarriers are monitored by time-resolved small-angle X-ray scattering (SAXS) coupled to a rapid-mixing stopped-flow technique. Nanoparticles (NPs) of nanochannel organization are formed by PEGylation, hydration, and dispersion of a lipid film of the fusogenic lipid monoolein in a mixture with positively charged (DOMA) and PEGylated (DOPE-PEG2000) amphiphiles and are characterized by the inner cubic structure of very large nanochannels favorable for DNA upload. Ultrafast structural dynamics of complexation and assembly of these cubosome particles with neurotrophic plasmid DNA (pDNA) is revealed thanks to the high brightness of the employed synchrotron X-ray beam. The rate constant of the pDNA/lipid NP complexation is estimated from dynamic roentgenograms recorded at 4 ms time resolution. pDNA upload into the vastly hydrated channels of the cubosome carriers leads to a fast nanoparticle\u2013nanoparticle structural transition and lipoplex formation involving tightly packed pDNA."@en . . "6"^^ . . . "4" . "Structural changes occurring on a millisecond time scale during uptake of DNA by cationic lipid nanocarriers are monitored by time-resolved small-angle X-ray scattering (SAXS) coupled to a rapid-mixing stopped-flow technique. Nanoparticles (NPs) of nanochannel organization are formed by PEGylation, hydration, and dispersion of a lipid film of the fusogenic lipid monoolein in a mixture with positively charged (DOMA) and PEGylated (DOPE-PEG2000) amphiphiles and are characterized by the inner cubic structure of very large nanochannels favorable for DNA upload. Ultrafast structural dynamics of complexation and assembly of these cubosome particles with neurotrophic plasmid DNA (pDNA) is revealed thanks to the high brightness of the employed synchrotron X-ray beam. The rate constant of the pDNA/lipid NP complexation is estimated from dynamic roentgenograms recorded at 4 ms time resolution. pDNA upload into the vastly hydrated channels of the cubosome carriers leads to a fast nanoparticle\u2013nanoparticle structural transition and lipoplex formation involving tightly packed pDNA." . "Lesieur, S." . "DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics"@en . "DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics"@en . "Angelov, Borislav" . "\u0160t\u011Bp\u00E1nek, Petr" . . . . "8"^^ . . "Angelov, Borislav" . "RIV/61389013:_____/13:00392998!RIV14-GA0-61389013" . "Filippov, Sergey" . . "DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics" . . "Journal of Physical Chemistry Letters" . . .