"7"^^ . "Hrom\u00E1dkov\u00E1, Ji\u0159ina" . "The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic .gamma.-Fe2O3 nanoparticles by mammalian cells"@en . . "112994" . . . . "Klyuchivska, O." . "Babi\u010D, Michal" . "13"^^ . . . . . "Zaso\u0144ska, Beata Anna" . "Hor\u00E1k, Daniel" . . . "I, P(7E12053), P(GAP206/12/0381)" . "maghemite; core-shell; nanoparticles"@en . "000316040500019" . . . "Gamma-Fe2O3 nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) chlorides with a base and subsequent oxidation were coated with a shell of hydrophilic biocompatible poly(N,N-dimethylacrylamide) (PDMAAm). Various initiators were attached to the iron oxide surface to enable the use of the \u201Cgrafting-from\u201C approach for immobilization of PDMAAm. They included 2,2-azobis(2-methylpropanimidamide) dihydrochloride (AMPA), 2,2-azobis(N-hydroxy-2-methylpropanimidamide) dihydrochloride (ABHA) and 4-cyano-4-{[1-cyano-3-(N-hydroxycarbamoyl)-1-methylpropyl]azo}pentanoic acid (CCHPA). Engulfment of PDMAAm-coated \u03B3-Fe2O3 nanoparticles by murine J774.2 macrophages was investigated. Only some nanoparticles were engulfed by the macrophages. PDMAAm-AMPA-\u03B3-Fe2O3 and PDMAAm-ABHA-\u03B3-Fe2O3 nanoparticles were rapidly engulfed by the cells. In contrast, neat \u03B3-Fe2O3 and PDMAAm-CCHPA-\u03B3-Fe2O3 particles induced formation of transparent vacuoles indicating toxicity of the particles. Thus, PDMAAm-coated AMPA- and ABHA-\u03B3-Fe2O3 nanoparticles can be recommended as non-toxic labels for mammalian cells." . "Boiko, N." . "Bene\u0161, Milan J." . . "The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic .gamma.-Fe2O3 nanoparticles by mammalian cells"@en . . . "Journal of Biomedical Nanotechnology" . . "Zaso\u0144ska, Beata Anna" . "RIV/61389013:_____/13:00391930!RIV14-MSM-61389013" . "Mackov\u00E1, Hana" . . . "The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic .gamma.-Fe2O3 nanoparticles by mammalian cells" . "10.1166/jbn.2013.1552" . "Stoika, R." . . "Trchov\u00E1, Miroslava" . . . "The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic .gamma.-Fe2O3 nanoparticles by mammalian cells" . "RIV/61389013:_____/13:00391930" . . "3" . "[BDDF696360AE]" . "1550-7033" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Gamma-Fe2O3 nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) chlorides with a base and subsequent oxidation were coated with a shell of hydrophilic biocompatible poly(N,N-dimethylacrylamide) (PDMAAm). Various initiators were attached to the iron oxide surface to enable the use of the \u201Cgrafting-from\u201C approach for immobilization of PDMAAm. They included 2,2-azobis(2-methylpropanimidamide) dihydrochloride (AMPA), 2,2-azobis(N-hydroxy-2-methylpropanimidamide) dihydrochloride (ABHA) and 4-cyano-4-{[1-cyano-3-(N-hydroxycarbamoyl)-1-methylpropyl]azo}pentanoic acid (CCHPA). Engulfment of PDMAAm-coated \u03B3-Fe2O3 nanoparticles by murine J774.2 macrophages was investigated. Only some nanoparticles were engulfed by the macrophages. PDMAAm-AMPA-\u03B3-Fe2O3 and PDMAAm-ABHA-\u03B3-Fe2O3 nanoparticles were rapidly engulfed by the cells. In contrast, neat \u03B3-Fe2O3 and PDMAAm-CCHPA-\u03B3-Fe2O3 particles induced formation of transparent vacuoles indicating toxicity of the particles. Thus, PDMAAm-coated AMPA- and ABHA-\u03B3-Fe2O3 nanoparticles can be recommended as non-toxic labels for mammalian cells."@en . . "9" . "10"^^ .