. . . . "Investigation of nanoparticle coating by fluorescence correlation spectroscopy"@en . . . "V\u00FDzkum nano\u010D\u00E1stic ve fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopii"@cs . "6"^^ . . "1022-1352" . "373212" . . "\u0160ubr, Vladim\u00EDr" . "\u0160t\u011Bp\u00E1nek, Petr" . "Jurkiewicz, Piotr" . "Ko\u0148\u00E1k, \u010Cestm\u00EDr" . . "RIV/61389013:_____/08:00311010!RIV09-AV0-61389013" . "RIV/61389013:_____/08:00311010" . . . "209" . "4"^^ . "Ulbrich, Karel" . "Macromolecular Chemistry and Physics" . . . . "Investigation of nanoparticle coating by fluorescence correlation spectroscopy"@en . . "14" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Protecting gene delivery vectors by hydrophilic copolymer coating is a promising approach in targeted gene therapy. Herein we demonstrate the usefulness of FCS in characterizing the coating process of a virus model - latex beads with coumarin-labeled N-(2-hydroxypropyl)methacrylamide copolymer containing thiazolidine-2-thione groups. FCS gives an estimation of the average number of polymer chains coating the latex bead, which agrees with light scattering results, giving an evidence of quality of the vector surface modification. FCS is advantageous because it decreases the sample consumption by a factor of 1 000. The coumarin label showed advantages over the previously used fluorescein-based one." . . "coatings; drug delivery systems; fluorescence; light scattering"@en . "Investigation of nanoparticle coating by fluorescence correlation spectroscopy" . . . "Investigation of nanoparticle coating by fluorescence correlation spectroscopy" . "P(IAA100500501), P(IAA4050403), P(KAN200200651), Z(AV0Z40400503), Z(AV0Z40500505)" . . "Hof, Martin" . "Protecting gene delivery vectors by hydrophilic copolymer coating is a promising approach in targeted gene therapy. Herein we demonstrate the usefulness of FCS in characterizing the coating process of a virus model - latex beads with coumarin-labeled N-(2-hydroxypropyl)methacrylamide copolymer containing thiazolidine-2-thione groups. FCS gives an estimation of the average number of polymer chains coating the latex bead, which agrees with light scattering results, giving an evidence of quality of the vector surface modification. FCS is advantageous because it decreases the sample consumption by a factor of 1 000. The coumarin label showed advantages over the previously used fluorescein-based one."@en . "7"^^ . "[229E235EC546]" . . . "V\u00FDzkum nano\u010D\u00E1stic ve fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopii"@cs . . . . "V\u00FDzkum nano\u010D\u00E1stic ve fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopii."@cs . "000258282400005" .