. "Ku\u010Dkov\u00E1, \u0160t\u011Bp\u00E1nka" . "572544" . "Hynek, R." . "Kod\u00ED\u010Dek, M." . . . "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings" . "Hradilov\u00E1, J." . . . . "P(GA203/04/2091), Z(AV0Z4032918), Z(MSM 223300006)" . "0951-4198" . "1896;1900" . "4"^^ . "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings" . "Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century." . "protein binders; mass spectrometry"@en . "18" . "1"^^ . "P\u0159\u00EDprava nanokrystalick\u00E9ho magnetitu vysokoenergetick\u00FDm mlet\u00EDm \u017Eeleza a hematitu"@cs . "P\u0159\u00EDprava nanokrystalick\u00E9ho magnetitu vysokoenergetick\u00FDm mlet\u00EDm \u017Eeleza a hematitu"@cs . "RIV/61388980:_____/04:00106530" . "RIV/61388980:_____/04:00106530!RIV/2005/AV0/A50005/N" . . . "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings"@en . "5"^^ . . . "17" . . "Rapid Communications in Mass Spectrometry" . . "Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century."@en . . . . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings"@en . "Magnetit byl p\u0159ipraven mlet\u00EDm mikro\u010D\u00E1stic hematitu a \u017Eeleza a pak studov\u00E1n chemickou anal\u00FDzou, elektronovou mikroskopi\u00ED, rtg difrakc\u00ED a Mossbauerovou spektroskopi\u00ED. Magnetit se vyzna\u010Doval velkou magnetickou tvrdost\u00ED a po dlouh\u00E9m mlet\u00ED obsahoval p\u0159\u00EDm\u011Bs wustitu (FeO)."@cs . "[673A8BAC2CB1]" .