"Inhibition of microbial \u03B2-N-acetylhexosaminidases by 4-deoxy- and galacto-analogues of NAG-thiazoline"@en . "RIV/61388971:_____/14:00435534!RIV15-GA0-61388971" . "RIV/61388971:_____/14:00435534" . "21849" . . "Bioorganic and Medicinal Chemistry Letters" . . "NAG-thiazoline is a well-established competitive inhibitor of two physiologically relevant glycosidase families\u2014\u03B2-N-acetylhexosaminidases (GH20) and \u03B2-N-acetylglucosaminidases (GH84). Based on the different substrate flexibilities of these enzyme groups, we designed and synthesized the 4-deoxy derivative of NAG-thiazoline aiming at the selective inhibition of GH20 \u03B2-N-acetylhexosaminidases. One GH84 and two GH20 microbial glycosidases were employed as model enzymes for the inhibition assays. Surprisingly, the new compound 4-deoxy-thiazoline exhibited no activity inhibition with either of the enzyme families of interest. Unlike with the substrates, the 4-hydroxyl group of the inhibitor\u2019s sugar ring seems to be crucial for binding the inhibitor to the active sites of these enzymes."@en . "000343901400039" . "Inhibition of microbial \u03B2-N-acetylhexosaminidases by 4-deoxy- and galacto-analogues of NAG-thiazoline" . "Sl\u00E1mov\u00E1, Krist\u00FDna" . "Pelantov\u00E1, Helena" . "Inhibition of microbial \u03B2-N-acetylhexosaminidases by 4-deoxy- and galacto-analogues of NAG-thiazoline"@en . "I, P(7E11011), P(GAP207/11/0629)" . "NAG-thiazoline is a well-established competitive inhibitor of two physiologically relevant glycosidase families\u2014\u03B2-N-acetylhexosaminidases (GH20) and \u03B2-N-acetylglucosaminidases (GH84). Based on the different substrate flexibilities of these enzyme groups, we designed and synthesized the 4-deoxy derivative of NAG-thiazoline aiming at the selective inhibition of GH20 \u03B2-N-acetylhexosaminidases. One GH84 and two GH20 microbial glycosidases were employed as model enzymes for the inhibition assays. Surprisingly, the new compound 4-deoxy-thiazoline exhibited no activity inhibition with either of the enzyme families of interest. Unlike with the substrates, the 4-hydroxyl group of the inhibitor\u2019s sugar ring seems to be crucial for binding the inhibitor to the active sites of these enzymes." . . . . "Inhibition of microbial \u03B2-N-acetylhexosaminidases by 4-deoxy- and galacto-analogues of NAG-thiazoline" . . "Kalachov\u00E1, Lubica" . . "22" . . . "[2875E60FA594]" . . . "Krejzov\u00E1, Jana" . . "K\u0159en, Vladim\u00EDr" . "24" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "NAG-thiazoline; enzyme inhibition"@en . "6"^^ . "\u0160imon, Petr" . . . . "3"^^ . . "6"^^ . . . "0960-894X" . .