. "17" . "14" . "Kapuscik, Alexandra" . . . . "Nov\u00E1k, Petr" . "I, P(ED2.1.00/03.0110), P(LH11129), V" . . . "RIV/61388971:_____/13:00424616" . "9"^^ . "Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent" . "000330181300016" . "Hrouzek, Pavel" . . . "Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesova 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-alpha-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC50 values of (3.51.5) mugmL(-1) ((4.01.7) muM) and (50.013.4) mugmL(-1) ((57.815.5) muM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-kappaB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect"@en . "Kuzma, Marek" . "Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesova 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-alpha-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC50 values of (3.51.5) mugmL(-1) ((4.01.7) muM) and (50.013.4) mugmL(-1) ((57.815.5) muM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-kappaB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect" . . "B\u00E1rtov\u00E1, Simona" . . . "Jokela, J." . . "92337" . "Eger, A." . "10"^^ . "Kapuscik, Alexandra" . "Pfluger, M." . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent"@en . "[390C58BE106B]" . "Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent" . "6"^^ . . . "1439-4227" . "Chembiochem" . . "NMR spectroscopy; anti-inflammatory; cyanobacteria"@en . . . "Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent"@en . "Hundsberger, H." . . . "10.1002/cbic.201300246" . . . . "Kopeck\u00FD, Ji\u0159\u00ED" . "RIV/61388971:_____/13:00424616!RIV14-MSM-61388971" .