"Wallner, T." . "Wilde, A." . . "Inactivation of the conserved open reading frame ycf34 of Synechocystis sp PCC 6803 interferes with the photosynthetic electron transport chain" . "NL - Nizozemsko" . "1817" . . . "RIV/61388971:_____/12:00389955" . "Reijerse, E. J." . "0005-2728" . . . . . "11"^^ . . . "Hagiwara, Y." . . "000309089400006" . "Ycf34 is a hypothetical chloroplast open reading frame that is present in the chloroplast genomes of several nongreen algae. Ycf34 homologues are also encoded in all sequenced genomes of cyanobacteria. To evaluate the role of Ycf34 we have constructed and analysed a cyanobacterial mutant strain. Inactivation of ycf34 in Synechocystis sp. PCC 6803 showed no obvious phenotype under normal light intensity growth conditions. However, when the cells were grown under low light intensity they contained less and smaller phycobilisome antennae and showed a strongly retarded growth, suggesting an essential role of the Ycf34 polypeptide under light limiting conditions. Northern blot analysis revealed a very weak expression of the phycocyanin operon in the ycf34 mutant under light limiting growth in contrast to the wild type and to normal light conditions. Oxygen evolution and P-700 measurements showed impaired electron flow between photosystem II and photosystem I under these conditions which suggest that the impaired antenna size is most likely due to a highly reduced plastoquinone pool which triggers regulation on a transcriptional level" . "[C061C65FC208]" . "Sobotka, Roman" . "Inactivation of the conserved open reading frame ycf34 of Synechocystis sp PCC 6803 interferes with the photosynthetic electron transport chain"@en . . "1"^^ . "I, P(ED2.1.00/03.0110)" . "141058" . . . . "Ycf34 is a hypothetical chloroplast open reading frame that is present in the chloroplast genomes of several nongreen algae. Ycf34 homologues are also encoded in all sequenced genomes of cyanobacteria. To evaluate the role of Ycf34 we have constructed and analysed a cyanobacterial mutant strain. Inactivation of ycf34 in Synechocystis sp. PCC 6803 showed no obvious phenotype under normal light intensity growth conditions. However, when the cells were grown under low light intensity they contained less and smaller phycobilisome antennae and showed a strongly retarded growth, suggesting an essential role of the Ycf34 polypeptide under light limiting conditions. Northern blot analysis revealed a very weak expression of the phycocyanin operon in the ycf34 mutant under light limiting growth in contrast to the wild type and to normal light conditions. Oxygen evolution and P-700 measurements showed impaired electron flow between photosystem II and photosystem I under these conditions which suggest that the impaired antenna size is most likely due to a highly reduced plastoquinone pool which triggers regulation on a transcriptional level"@en . "7"^^ . "Frankenberg-Dinkel, N." . . . "Bern\u00E1t, G." . "Hypothetical chloroplast open reading frame; Iron-sulphur protein; Phycobilisome"@en . . "Biochimica Et Biophysica Acta-Bioenergetics" . "11" . "RIV/61388971:_____/12:00389955!RIV13-AV0-61388971" . "Inactivation of the conserved open reading frame ycf34 of Synechocystis sp PCC 6803 interferes with the photosynthetic electron transport chain"@en . "Inactivation of the conserved open reading frame ycf34 of Synechocystis sp PCC 6803 interferes with the photosynthetic electron transport chain" .