. . . "High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli"@en . . "RIV/61388971:_____/11:00369717" . . "Van\u011Bk, Ond\u0159ej" . "High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli" . . . . . "Natural killer cell; NKR-P1C receptor; NK1.1 antigen"@en . . . "Bezou\u0161ka, Karel" . "Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain"@en . "7"^^ . "Kavan, Daniel" . "High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli"@en . . . "Rozbesk\u00FD, Daniel" . . "Protein Expression and Purification" . "202069" . . "2" . . . . "Chmel\u00EDk, Josef" . "000288591900008" . "P(1M0505), P(GA303/09/0477), P(GAP207/10/1040), P(GD305/09/H008), P(IAA500200620), P(LC07017), P(LC545), S, Z(AV0Z50200510), Z(MSM0021620808)" . . . "Nov\u00E1k, Petr" . . "6"^^ . "77" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "6"^^ . "Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain" . . "[42B1EBF70FE3]" . "High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli" . . . . . . . "RIV/61388971:_____/11:00369717!RIV12-AV0-61388971" . "10.1016/j.pep.2011.01.013" . . . . "1046-5928" .