"1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) byl pou\u017Eit k aktivaci postrann\u00EDch karboxylov\u00FDch skupin aminokyselin (kyseliny glutamov\u00E1, asparagov\u00E1 a C-konec) pro zes\u00ED\u0165ov\u00E1c\u00ED reakci. Jakmile je karboxylov\u00FD zbytek aktivovan\u00FD tvo\u0159\u00ED \u201Enulov\u00E9\u201C zes\u00EDt\u011Bn\u00ED (lidick\u00E1 vazba) s bl\u00EDzkou aminoskupinou (lysin a N konec). Takto aktivovan\u00E9karboxylov\u00E9 zbytky tak\u00E9 m\u016F\u017Eou v p\u0159\u00EDtomnosti dihydrazid\u016F o r\u016Fzn\u00FDch velikostech tvo\u0159it zes\u00EDt\u011Bn\u00ED mezi sebou"@cs . . . "1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was used to activate the carboxylic acid containing residues, aspartic acid (D), glutamic acid (E), and the carboxy terminus (O), for cross-linking reactions. Once activated, the DEO sidechains can react to form \u201Czero-length cross-links with nearby primary amine containing residues, lysines (K) and the amino terminus (X), via the formation of a new amide bond. Also the EDC-activated DEO sidechains can be cross-linked to each other using dihydrazides, two hydrazide moieties connected by an alkyl cross-linker arm of variable length"@en . . . "Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "proteins; top-down; ft-ms"@en . . "373080" . "RIV/61388971:_____/08:00320965" . "14" . "[EDB224D26982]" . "000262813100004" . "RIV/61388971:_____/08:00320965!RIV09-AV0-61388971" . . "1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was used to activate the carboxylic acid containing residues, aspartic acid (D), glutamic acid (E), and the carboxy terminus (O), for cross-linking reactions. Once activated, the DEO sidechains can react to form \u201Czero-length cross-links with nearby primary amine containing residues, lysines (K) and the amino terminus (X), via the formation of a new amide bond. Also the EDC-activated DEO sidechains can be cross-linked to each other using dihydrazides, two hydrazide moieties connected by an alkyl cross-linker arm of variable length" . . . . "1"^^ . "Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies" . "2"^^ . "P(LC545), Z(AV0Z50200510)" . "11"^^ . . "Nov\u00E1k, Petr" . "Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies"@en . "Vyu\u017Eit\u00ED intramolekul\u00E1rn\u00EDho zes\u00EDt\u011Bn\u00ED postrann\u00EDch karboxylov\u00FDch skupin pro studium struktury protein\u016F.."@cs . . . "Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies"@en . "European Journal of Mass Spectrometry" . . . "1469-0667" . . "Kruppa, G. H." . "6" . . "Vyu\u017Eit\u00ED intramolekul\u00E1rn\u00EDho zes\u00EDt\u011Bn\u00ED postrann\u00EDch karboxylov\u00FDch skupin pro studium struktury protein\u016F.."@cs .