"A home-made nano-desorption electrospray ionization (nano-DESI) device and the kinetic method were tested in chiral analysis of model clinical samples containing enantiomers of one of three pharmaceutically important compounds: dihydroxyphenylalanine (DOPA), ephedrine and ibuprofen. The initial evaluation of chiral systems was carried out by direct infusion of solution mixtures (analyte/central metal/chiral reference ligand) to a standard electrospray ionization (ESI) source. Cu(II) was used as a central metal for all analytes, L-phenylalanine was applied as a chiral reference ligand for DOPA, whereas L-tryptophan was used for the other two analytes. Then, the ESI source was substituted by a nano-DESI source and dried spots of 1 \u03BCL samples of whole human blood spiked with individual drugs were successfully analyzed without any pre-treatment" . "P(GA203/07/0765), P(LC545), Z(AV0Z50200510)" . . . . "RIV/61388971:_____/08:00320962" . "Bedn\u00E1\u0159, P." . "A home-made nano-desorption electrospray ionization (nano-DESI) device and the kinetic method were tested in chiral analysis of model clinical samples containing enantiomers of one of three pharmaceutically important compounds: dihydroxyphenylalanine (DOPA), ephedrine and ibuprofen. The initial evaluation of chiral systems was carried out by direct infusion of solution mixtures (analyte/central metal/chiral reference ligand) to a standard electrospray ionization (ESI) source. Cu(II) was used as a central metal for all analytes, L-phenylalanine was applied as a chiral reference ligand for DOPA, whereas L-tryptophan was used for the other two analytes. Then, the ESI source was substituted by a nano-DESI source and dried spots of 1 \u03BCL samples of whole human blood spiked with individual drugs were successfully analyzed without any pre-treatment"@en . "Desorp\u010Dn\u00ED nanoelektrosprej a kinetick\u00E1 metoda v chir\u00E1ln\u00ED anal\u00FDze klinick\u00FDch vzork\u016F"@cs . "European Journal of Mass Spectrometry" . "1469-0667" . . . "6" . "nano-desorption electrospray; mass spectrometry; kinetic method"@en . . "Pomoc\u00ED kinetick\u00E9 metody a elektrospreje byla odlad\u011Bna kvalitativn\u00ED a kvantivativn\u00ED stanoven\u00ED t\u0159\u00ED v\u00FDznamn\u00FDch farmaceutick\u00FDch substanc\u00ED: dihydroxyphenylalaninu (DOPA), ephedrinu and ibuprofenu. P\u0159i tvorb\u011B tern\u00E1rn\u00EDho komplexu, jeho\u017E disociace byla pou\u017Eita k odli\u0161en\u00ED D a L-forem, byla pou\u017Eita dvojmocn\u00E1 m\u011B\u010F a L-fenylalanin jako chir\u00E1ln\u00ED ligand. D\u00E1le byl postaven iontov\u00FD zdroj, kter\u00FD pracuje na principu desorp\u010Dn\u00EDho nanoelektrospreje. Na n\u011Bm byla enantioselektivn\u00ED stanoven\u00ED v\u00FD\u0161e uveden\u00FDch l\u00E1tek provedena. Bylo demonstrov\u00E1no, \u017Ee stanoven\u00ED lze prov\u00E9st i z lidsk\u00E9 krve. Uveden\u00FD postup m\u016F\u017Ee b\u00FDt pou\u017Eit k chir\u00E1ln\u00ED anal\u00FDze l\u00E9\u010Div obecn\u011B"@cs . "Lemr, Karel" . "Nano-desorption electrospray and kinetic method in chiral analysis of clinical samples" . . . . "Nano-desorption electrospray and kinetic method in chiral analysis of clinical samples" . "Nano-desorption electrospray and kinetic method in chiral analysis of clinical samples"@en . "14" . "7"^^ . . "Havl\u00ED\u010Dek, Vladim\u00EDr" . . "381593" . . . "RIV/61388971:_____/08:00320962!RIV09-AV0-61388971" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "2"^^ . . "000262813100009" . "4"^^ . "[34FD429161D3]" . . "Desorp\u010Dn\u00ED nanoelektrosprej a kinetick\u00E1 metoda v chir\u00E1ln\u00ED anal\u00FDze klinick\u00FDch vzork\u016F"@cs . "Ranc, V." . . . "Nano-desorption electrospray and kinetic method in chiral analysis of clinical samples"@en . .