"Vejvoda, Vojt\u011Bch" . "1381-1177" . "Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4"@en . . . . "Journal of Molecular Catalysis B-Enzymatic" . . . . . . "[466C5E5F3902]" . "NL - Nizozemsko" . "Mart\u00EDnkov\u00E1, Ludmila" . "K\u0159en, Vladim\u00EDr" . "Sl\u00E1mov\u00E1, Krist\u00FDna" . "Pelantov\u00E1, Helena" . "Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4" . . "Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4"@en . "P(GA203/05/2267), P(LC06010), P(OC 171), Z(AV0Z50200510)" . . "van Pelt, S." . "358224" . "Gallienne, E." . . . "\u010C\u00E1ste\u010Dn\u011B purifikovan\u00E1 nitrilhydratasa z Rhodococcus erythropolis A4 byla pou\u017Eita pro biotransformace r\u016Fzn\u00FDch alkyl- a acyl-nitril\u016F na amidy. Enzymov\u00E9 prepar\u00E1ty s nitrilhydratasovou a amidasovou aktivitou byly p\u0159ipraveny a pou\u017Eity pro hydrataci nitril\u016F v p\u0159\u00EDtomnosti s\u00EDranu amonn\u00E9ho, kter\u00FD selektivn\u011B inhibuje amidasovou aktivitu. Geny nha1 a nha2 k\u00F3duj\u00EDc\u00ED alfa a beta podjednotky nitrilhydratasy byly klonov\u00E1ny a sekvenov\u00E1ny"@cs . "7"^^ . . "Bolte, J." . "2-4" . "Lutz-Wahl, S." . "Kub\u00E1\u010D, David" . "rhodococcus erythropolis; nitrile hydratase; amidase"@en . "Eli\u0161\u00E1kov\u00E1, Veronika" . "Fischer, L." . . . . "17"^^ . "RIV/61388971:_____/08:00320846" . . "10"^^ . "Lemaire, M." . "P\u00E1tek, Miroslav" . . "Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4" . "Kuzma, Marek" . "Kaplan, Ond\u0159ej" . . . . "Biotransformace nitril\u016F na amidy s pou\u017Eit\u00EDm rozpustn\u00E9 a imobilizovan\u00E9 nitrilhydratasy z Rhodococcus erythropolis A4"@cs . . . . "A semi-purified nitrile hydratase from Rhodococcus erythropolis A4 was applied to biotransformations of various alkyl- and acyl-nitriles into amides. Enzyme preparates with nitrile hydratase and amidase activities were prepared and used for nitrile hydration in presence of ammonium sulfate, which selectively inhibited amidase activity. The genes nha1 and nha2 coding for alpha and beta subunits of nitrile hydratase were cloned and sequenced" . "Biotransformace nitril\u016F na amidy s pou\u017Eit\u00EDm rozpustn\u00E9 a imobilizovan\u00E9 nitrilhydratasy z Rhodococcus erythropolis A4"@cs . "50" . "000252487300010" . . . . . "T\u00F3thov\u00E1, A." . "A semi-purified nitrile hydratase from Rhodococcus erythropolis A4 was applied to biotransformations of various alkyl- and acyl-nitriles into amides. Enzyme preparates with nitrile hydratase and amidase activities were prepared and used for nitrile hydration in presence of ammonium sulfate, which selectively inhibited amidase activity. The genes nha1 and nha2 coding for alpha and beta subunits of nitrile hydratase were cloned and sequenced"@en . "RIV/61388971:_____/08:00320846!RIV09-AV0-61388971" .