. . "Weiserov\u00E1, Marie" . "Restrik\u010Dn\u011B \u2013 modifika\u010Dn\u00ED enzym Typu I EcoR124I je slo\u017Een ze t\u0159\u00ED podjednotek v pom\u011Bru HsdR2:HsdM2:HsdS1. Podjednotka HsdR je p\u0159\u00EDkladem archetypu fuse mezi nukleasovou a helikasovou dom\u00E9nou do jednoho polypeptidu. Zkoumali jsme vztah mezi t\u011Bmito fyzik\u00E1ln\u011B v\u00E1zan\u00FDmi dom\u00E9nami anal\u00FDzou DNA transloka\u010Dn\u00EDch vlastnost\u00ED komplexu EcoR124I, kde HsdR podjednotky nesly mutace v RecB-nucleasov\u00E9m motivu II nebo III. Mutace v nukleasov\u00E9 dom\u00E9n\u011B ovliv\u0148uj\u00ED translokaci DNA p\u0159esto\u017Ee jsou od helikasov\u00E9 dom\u00E9ny odd\u011Bleny. Krom\u011B redukce restrik\u010Dn\u00ED aktivity jsme tak\u00E9 pozorovali sn\u00ED\u017Eenou rychlost translokace a ATPasov\u00E9 aktivity; rozd\u00EDln\u00E9 populace enzym\u016F s rozd\u00EDln\u00FDmi charakteristikami transloka\u010Dn\u00ED rychlosti; tendenci k zastavov\u00E1n\u00ED b\u011Bhem iniciace translokace; a zm\u011Bn\u011Bnou dynamiku turnoveru HsdR"@cs . . "The Type I Restriction-Modification enzyme EcoR124I comprises three subunits with the stoichiometry HsdR2:HsdM2:HsdS1. The HsdR subunits are archetypical examples of the fusion between nuclease and helicase domains into a single polypeptide, a linkage that is found in a great many other DNA processing enzymes. To explore the interrelationship between these physically-linked domains, we examined the DNA translocation properties of EcoR124I complexes in which the HsdR subunits had been mutated in the RecB-like nuclease motifs II or III. We found that nuclease mutations could have noteworthy effects on DNA translocation despite being discrete from the helicase domain. In addition to reductions in DNA cleavage activity, we also observed: decreased translocation and ATPase rates; different enzyme populations with different characteristic translocation rates; a tendency to stall during initiation; and, altered HsdR turnover dynamics"@en . "0022-2836" . . . . "2"^^ . "384" . . "single molecule; enzyme disorder; translocase"@en . "\u0160i\u0161\u00E1kov\u00E1, Eva" . "Szczelkun, M. D." . . . . . "Vztah helikasov\u00E9 a nukleasov\u00E9 dom\u00E9ny b\u011Bhem translokace DNA molekul\u00E1rn\u00EDm motorem EcoR124I"@cs . . . "Dekker, C." . "P(GA204/07/0325), Z(AV0Z50200510)" . "RIV/61388971:_____/08:00317626!RIV09-AV0-61388971" . "The Type I Restriction-Modification enzyme EcoR124I comprises three subunits with the stoichiometry HsdR2:HsdM2:HsdS1. The HsdR subunits are archetypical examples of the fusion between nuclease and helicase domains into a single polypeptide, a linkage that is found in a great many other DNA processing enzymes. To explore the interrelationship between these physically-linked domains, we examined the DNA translocation properties of EcoR124I complexes in which the HsdR subunits had been mutated in the RecB-like nuclease motifs II or III. We found that nuclease mutations could have noteworthy effects on DNA translocation despite being discrete from the helicase domain. In addition to reductions in DNA cleavage activity, we also observed: decreased translocation and ATPase rates; different enzyme populations with different characteristic translocation rates; a tendency to stall during initiation; and, altered HsdR turnover dynamics" . "[ECCF815734AF]" . . . "The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I"@en . "Journal of Molecular Biology" . "14"^^ . "The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I"@en . . "Vztah helikasov\u00E9 a nukleasov\u00E9 dom\u00E9ny b\u011Bhem translokace DNA molekul\u00E1rn\u00EDm motorem EcoR124I"@cs . "Seidel, R." . "5" . "373033" . "The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I" . "5"^^ . . "000262016600021" . "RIV/61388971:_____/08:00317626" . .